{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T15:42:06Z","timestamp":1763480526148,"version":"3.41.2"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T00:00:00Z","timestamp":1651795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Chagas disease (CD), caused by the hemoflagellate protozoan <jats:italic>Trypanosoma cruzi<\/jats:italic>, affects more than six million people worldwide and presents an unsatisfactory therapy, based on two nitroderivatives, introduced in clinical medicine for decades. The synthetic peptide, with CTHRSSVVC sequence (PepA), mimics the CD163 and TNF-\u03b1 tripeptide \u201cRSS\u201d motif and binds to atheromatous plaques in carotid biopsies of human patients, spleen tissues, and a low-density lipoprotein receptor knockout (LDLr\u2212\/\u2212) mouse model of atherosclerosis. CD163 receptor is present on monocytes, macrophages, and neutrophils, acting as a regulator of acute-phase processes and modulating aspects of the inflammatory response and the establishment of infections. Due to the potential theranostic role of PepA, our aim was to investigate its effect upon <jats:italic>T. cruzi<\/jats:italic> infection <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. PepA and two other peptides with shuffled sequences were assayed upon different binomials of host cell\/parasite, including professional [as peritoneal mouse macrophages (PMM)] and non-professional phagocytes [primary cultures of cardiac cells (CM)], under different protocols. Also, their impact was further addressed <jats:italic>in vivo<\/jats:italic> using a mouse model of acute experimental Chagas disease. Our <jats:italic>in-vitro<\/jats:italic> findings demonstrate that PepA and PepB (the peptide with random sequence retaining the \u201cRS\u201d sequence) reduced the intracellular parasitism of the PMM but were inactive during the infection of cardiac cells. Another set of <jats:italic>in-vitro<\/jats:italic> and <jats:italic>in-vivo<\/jats:italic> studies showed that they do not display a trypanocidal effect on bloodstream trypomastigotes nor exhibit <jats:italic>in-vivo<\/jats:italic> efficacy when administered after the parasite inoculation. Our data report the <jats:italic>in-vitro<\/jats:italic> activity of PepA and PepB upon the infection of PMM by <jats:italic>T. cruzi<\/jats:italic>, possibly triggering the microbicidal arsenal of the host professional phagocytes, capable of controlling parasitic invasion and proliferation.<\/jats:p>","DOI":"10.3389\/fcimb.2022.882555","type":"journal-article","created":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T07:50:02Z","timestamp":1651823402000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["The Impact of the CTHRSSVVC Peptide Upon Experimental Models of Trypanosoma cruzi Infection"],"prefix":"10.3389","volume":"12","author":[{"given":"Gabriela Rodrigues","family":"Leite","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise da Gama Ja\u00e9n","family":"Batista","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Lia","family":"Mazzeti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosemeire Aparecida","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ademar Ben\u00e9volo","family":"Lug\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria de Nazar\u00e9 Correia","family":"Soeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,5,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2940","DOI":"10.1128\/AAC.01617-09","article-title":"Arylimidamide DB766, A Potential Chemotherapeutic Candidate for Chagas\u2019 Disease Treatment","volume":"54","author":"Batista","year":"2010","journal-title":"Antimicrobial Agents Chemother"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/tid.12783","article-title":"An Autopsy-Based Study of Trypanosoma Cruzi Persistence in Organs of Chronic Chagasic Patients and its Relevance for Transplantation","volume":"19","author":"Benvenuti","year":"2017","journal-title":"Transplant. 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