{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T04:13:30Z","timestamp":1793506410977,"version":"4.5.0"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Nocardia<\/jats:italic> is an opportunistic pathogen that mainly involves immunosuppressed patients and causes a high mortality rate. As an emerging approach to detect infectious pathogens, metagenomic next-generation sequencing (mNGS) was reported in the detection of <jats:italic>Nocardia<\/jats:italic>. However, there is no evidence demonstrating the effect of mNGS on the prognosis of <jats:italic>Nocardia<\/jats:italic> infection. In this retrospective study, we included 18 nocardiosis patients. <jats:italic>Nocardia<\/jats:italic> species were detected by mNGS from their clinical samples. All the patients were diagnosed with nocardiosis by clinical experts through a comprehensive evaluation. Of these 18 patients, fever is the most frequent initial symptom. Compared to traditional culture methods, mNGS provides a faster turnaround time (TAT) and higher sensitivity. Pulmonary nocardiosis was the most common clinical presentation in the study. mNGS detected 13 types of <jats:italic>Nocardia<\/jats:italic> species, of which <jats:italic>Nocardia abscessus<\/jats:italic> and <jats:italic>Nocardia cyriacigeorgica<\/jats:italic> were the most common species. The study\u2019s most noteworthy discovery is that mNGS outperforms culture at detecting mixed infections (more than one pathogen detected in one clinical specimen, including bacteria, fungi, and excluding virus), and number of infectious species was an independent risk factor for nocardiosis patients\u2019 prognostics after adjusting age, ICU days, gender and underlying diseases (adjusted HR = 1.47, 95% CI: 1.09-1.98, p = 0.011). As a result, we believe that by detecting mixed infections (more than one pathogenic species), mNGS can provide a clinical risk warning for the prognosis of nocardiosis.<\/jats:p>","DOI":"10.3389\/fcimb.2022.894678","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T07:25:47Z","timestamp":1661930747000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":26,"title":["Metagenomic next-generation sequencing provides prognostic warning by identifying mixed infections in nocardiosis"],"prefix":"10.3389","volume":"12","author":[{"given":"Mengfan","family":"Jiao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxu","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoguang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhu","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1007\/s15010-009-9193-9","article-title":"Nocardiosis: updated clinical review and experience at a tertiary center","volume":"38","author":"Ambrosioni","year":"2010","journal-title":"Infection"},{"key":"B2","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1093\/infdis\/134.3.286","article-title":"Nocardial infections in the united states 1972\u20131974","volume":"134","author":"Beaman","year":"1976","journal-title":"J. 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