{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T06:26:48Z","timestamp":1784010408073,"version":"3.55.0"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Porphyromonas gingivalis<\/jats:italic> is an important oral pathogen that causes periodontal disease and is difficult to culture under conventional conditions. Therefore, a reliable technique for detecting this pathogenic bacterium is required. Here, isothermal recombinase polymerase amplification (RPA), a new nucleic acid amplification method, was combined with a visualization method based on nanoparticle-based lateral flow strips (LFS) for the rapid detection of <jats:italic>P. gingivalis<\/jats:italic>. The species-specific 16S rRNA sequence of <jats:italic>P. gingivalis<\/jats:italic> was used as the target for RPA, and a set of specific primer\u2013probe combinations were designed and screened to amplify the target sequences. As a thermostatic amplification method, the RPA reaction, under optimized conditions, takes only 30\u00a0min to complete at a constant temperature (37\u00b0C). The amplification reaction products can be detected visually by LFS without any need for special equipment. The RPA-LFS method established for the detection of <jats:italic>P. gingivalis<\/jats:italic> was shown to be highly specific in distinguishing <jats:italic>P. gingivalis<\/jats:italic> from other pathogenic organisms by using 20 clinical isolates of <jats:italic>P. gingivalis<\/jats:italic> and 23 common pathogenic microorganisms. Susceptibility measurements and probit regression analysis were performed with gradient dilutions of <jats:italic>P. gingivalis<\/jats:italic> genomic DNA. The method was obtained to be highly sensitive, with a detection limit of 9.27 CFU per reaction at 95% probability. By analyzing the gingival sulcus fluid specimens from 130 patients with chronic periodontitis, the results showed that the RPA-LFS method detected 118 positive cases and 12 negative cases of <jats:italic>P. gingivalis<\/jats:italic>, and the results obtained were consistent with those of a conventional PCR assay. The RPA\u2013LFS method is an efficient, rapid, and convenient diagnostic method that simplifies the tedious process of detecting <jats:italic>P. gingivalis<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fcimb.2022.895261","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T07:09:05Z","timestamp":1653462545000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Fast, Simple, and Highly Specific Molecular Detection of Porphyromonas gingivalis Using Isothermal Amplification and Lateral Flow Strip Methods"],"prefix":"10.3389","volume":"12","author":[{"given":"Duobao","family":"Ge","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bendi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuzhu","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenxing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1007\/s00604-018-2677-x","article-title":"On Site Visual Detection of Porphyromonas Gingivalis Related Periodontitis by Using a Magnetic-Nanobead Based Assay for Gingipains Protease Biomarkers","volume":"185","author":"Alhogail","year":"2018","journal-title":"Mikrochim Acta"},{"key":"B2","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.archoralbio.2018.11.025","article-title":"Detection and Quantification of Porphyromonas Gingivalis and Aggregatibacter Actinomycetemcomitans in Bacteremia Induced by Interdental Brushing in Periodontally Healthy and Periodontitis Patients","volume":"98","author":"Ambrosio","year":"2019","journal-title":"Arch. 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