{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T22:32:02Z","timestamp":1767997922464,"version":"3.49.0"},"reference-count":122,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T00:00:00Z","timestamp":1657152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Zika is a vector-borne disease caused by an arbovirus (ZIKV) and overwhelmingly transmitted by <jats:italic>Ae. aegypti<\/jats:italic>. This disease is linked to adverse fetal outcomes, mostly microcephaly in newborns, and other clinical aspects such as acute febrile illness and neurologic complications, for example, Guillain-Barr\u00e9 syndrome. One of the most promising strategies to mitigate arbovirus transmission involves releasing <jats:italic>Ae. aegypti<\/jats:italic> mosquitoes carrying the maternally inherited endosymbiont bacteria <jats:italic>Wolbachia pipientis<\/jats:italic>. The presence of <jats:italic>Wolbachia<\/jats:italic> is associated with a reduced susceptibility to arboviruses and a fitness cost in mosquito life-history traits such as fecundity and fertility. However, the mechanisms by which <jats:italic>Wolbachia<\/jats:italic> influences metabolic pathways leading to differences in egg production remains poorly known. To investigate the impact of coinfections on the reproductive tract of the mosquito, we applied an isobaric labeling-based quantitative proteomic strategy to investigate the influence of <jats:italic>Wolbachia w<\/jats:italic>Mel and ZIKV infection in <jats:italic>Ae. aegypti<\/jats:italic> ovaries. To the best of our knowledge, this is the most complete proteome of <jats:italic>Ae. aegypti<\/jats:italic> ovaries reported so far, with a total of 3913 proteins identified, were also able to quantify 1044 <jats:italic>Wolbachia<\/jats:italic> proteins in complex sample tissue of <jats:italic>Ae. aegypti<\/jats:italic> ovary. Furthermore, from a total of 480 mosquito proteins modulated in our study, we discuss proteins and pathways altered in <jats:italic>Ae. aegypti<\/jats:italic> during ZIKV infections, <jats:italic>Wolbachia<\/jats:italic> infections, coinfection <jats:italic>Wolbachia<\/jats:italic>\/ZIKV, and compared with no infection, focusing on immune and reproductive aspects of <jats:italic>Ae. aegypti<\/jats:italic>. The modified aspects mainly were related to the immune priming enhancement by <jats:italic>Wolbachia<\/jats:italic> presence and the modulation of the Juvenile Hormone pathway caused by both microorganism\u2019s infection.<\/jats:p>","DOI":"10.3389\/fcimb.2022.900608","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T05:01:28Z","timestamp":1657170088000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary of Aedes aegypti Coinfected With ZIKV and Wolbachia"],"prefix":"10.3389","volume":"12","author":[{"given":"Lu\u00eds Felipe Costa","family":"Ramos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jimmy Rodriguez","family":"Murillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilberto Barbosa","family":"Domont","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle Maria Perp\u00e9tua","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1bio C\u00e9sar Sousa","family":"Nogueira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Maciel-de-Freitas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magno","family":"Junqueira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,7,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1016\/j.tibs.2015.08.001","article-title":"Helicases in Antiviral Immunity: Dual Properties as Sensors and Effectors","volume":"40","author":"Ahmad","year":"2015","journal-title":"Trends Biochem. 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