{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:49:37Z","timestamp":1753890577538,"version":"3.41.2"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T00:00:00Z","timestamp":1659398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K08818"],"award-info":[{"award-number":["JP20K08818"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20fk0108071"],"award-info":[{"award-number":["JP20fk0108071"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Ciprofloxacin (CIP) is a commonly used antibiotic for meningococcal chemoprophylaxis, and the mutations in the quinolone resistance-determining region of <jats:italic>gyrA<\/jats:italic> are associated with CIP-resistant <jats:italic>Neisseria meningitidis<\/jats:italic>. Here, we established a mismatched PCR-restriction fragment length polymorphism (RFLP) assay to detect a mutation at codon 91 of <jats:italic>gyrA<\/jats:italic>, followed by high-level CIP-resistant meningococci. We designed PCR-RFLP primers to detect the T91I mutation in <jats:italic>gyrA<\/jats:italic> by introducing an artificial <jats:italic>Aci<\/jats:italic>I cleavage site. This assay was performed using 26\u00a0<jats:italic>N. meningitidis<\/jats:italic> strains whose <jats:italic>gyrA<\/jats:italic> sequences have been characterized. The amplified 160 bp PCR product from <jats:italic>gyrA<\/jats:italic> was digested into three fragments (80, 66, and 14 bp) when there was no mutation, or two fragments (146 and 14 bp) when there was a mutation at codon 91.\u00a0A correlation was observed between the mismatched PCR-RFLP assay and <jats:italic>gyrA<\/jats:italic> sequencing. This rapid, simple, and accurate assay has the potential to detect CIP-resistant <jats:italic>N. meningitidis<\/jats:italic> in clinical microbiology laboratories, contributing to the appropriate antibiotic selection for meningococcal chemoprophylaxis, will help maintain an effective treatment for close contacts of IMD patients, and prevent the spread of CIP-resistant <jats:italic>N. meningitidis<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fcimb.2022.911911","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T04:35:54Z","timestamp":1659414954000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular detection of fluoroquinolone-resistant Neisseria meningitidis by using mismatched PCR-restriction fragment length polymorphism technique"],"prefix":"10.3389","volume":"12","author":[{"given":"Yusuke","family":"Ota","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reina","family":"Okada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideyuki","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoichi","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1093\/jac\/dkh075","article-title":"Neisseria meningitidis showing decreased susceptibility to ciprofloxacin: first report in Spain","volume":"53","author":"Alcala","year":"2004","journal-title":"J. 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