{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T05:45:07Z","timestamp":1768887907233,"version":"3.49.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T00:00:00Z","timestamp":1657497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>The zoonotic protozoa parasites <jats:italic>Cryptosporidium<\/jats:italic> spp. and <jats:italic>Giardia duodenalis<\/jats:italic> infect a wide range of hosts, including humans. Pigs are reservoir hosts for <jats:italic>Cryptosporidium<\/jats:italic> spp. and <jats:italic>G. duodenalis<\/jats:italic>, which can transmit cryptosporidiosis and giardiasis to humans and other animals. The current study sought to investigate the infection rates and species\/genotypes of <jats:italic>Cryptosporidium<\/jats:italic> spp. and <jats:italic>G. duodenalis<\/jats:italic> in pigs in Hubei of China. The nested PCR and sequence analyses of the small ribosomal subunit RNA (<jats:italic>SSU<\/jats:italic> rRNA) gene for <jats:italic>Cryptosporidium<\/jats:italic> spp. and the \u03b2-giardin (bg) gene for <jats:italic>G. duodenalis<\/jats:italic> was utilized to screen for the infection of those parasites in a total of 826 fresh fecal samples. Both <jats:italic>Cryptosporidium<\/jats:italic> spp. and <jats:italic>G. duodenalis<\/jats:italic> infection rates were 0.97% (8\/826). Of the genotyped positive isolates, 6\/8 (75%) were <jats:italic>C. scrofarum<\/jats:italic> and 2\/8 (25%) were <jats:italic>C. suis<\/jats:italic>, while two zoonotic species <jats:italic>G. duodenalis<\/jats:italic> assemblage E and assemblage A were also detected in 7\/8 (87.5%) isolates and 1\/8 (12.5%) isolates, respectively. The findings suggest that both of those parasites in pig in intensive farms of Hubei province, China, pose a potential public health risk.<\/jats:p>","DOI":"10.3389\/fcimb.2022.949773","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T04:50:53Z","timestamp":1657515053000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["First Characterization and Zoonotic Potential of Cryptosporidium spp. and Giardia duodenalis in Pigs in Hubei Province of China"],"prefix":"10.3389","volume":"12","author":[{"given":"Dongfang","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaxin","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junlong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,7,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1016\/j.exppara.2009.01.008","article-title":"Giardia Genotypes in Pigs in Western Australia: Prevalence and Association With Diarrhea","volume":"121","author":"Armson","year":"2009","journal-title":"Exp. 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