{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T16:24:29Z","timestamp":1783700669394,"version":"3.55.0"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T00:00:00Z","timestamp":1665446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018553","name":"Science and Technology Plan Project of Wenzhou, China","doi-asserted-by":"publisher","award":["32002312, LQ21H160001, Y20210080 , Y2020103"],"award-info":[{"award-number":["32002312, LQ21H160001, Y20210080 , Y2020103"]}],"id":[{"id":"10.13039\/501100018553","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>To exploit the <jats:italic>Rhinolophus sinicus<\/jats:italic>\u2013specific virome, 29 <jats:italic>Rhinolophus sinicus<\/jats:italic> were gathered in Lincang, China. Enriched viral sequences of 22 virus families were acquired by metavirome techniques. Hereby, the part of virome in <jats:italic>Rhinolophus sinicus<\/jats:italic>, including Chikungunya virus (CHIKV), Getah virus, and Japanese encephalitis virus (JEV) were validated by PCR. Five CHIKV viral sequences were amplified, among which CHIKV-China\/B2016C-1 shared the highest homology to CHIKV isolated from Italy in 2007, with the genotype as African ECS. Eight JEV viral sequences were amplified, of which JEV-China\/B2016E-1 shared the highest homology with at least 91.3% nt identity with the JEV sequence found in South Korea in 1988 and was classified as genotype III. Notably, JEV was isolated for the first time in <jats:italic>Rhinolophus sinicus<\/jats:italic>. The newly isolated JEV-China\/B2016-1 could increase infectivity while passaging in Vero cells from BHK-21 cells. Overall, the research sheds insight into the diversity and viral susceptibility dynamics of the virome in <jats:italic>Rhinolophus sinicus<\/jats:italic> and reveals new light on the ecology of other important viral hosts.<\/jats:p>","DOI":"10.3389\/fcimb.2022.960507","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T07:12:26Z","timestamp":1665472346000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses"],"prefix":"10.3389","volume":"12","author":[{"given":"Chengcheng","family":"Peng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiquan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengpeng","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.virol.2021.05.008","article-title":"Analysis of codon usage of horseshoe bat hepatitis b virus and its host","volume":"561","author":"Bornali","year":"2021","journal-title":"Virology."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph14101227","article-title":"Behaviors related to mosquito-borne diseases among different ethnic minority groups along the China-Laos border areas","volume":"14","author":"Chao","year":"2017","journal-title":"Int. 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