{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T19:05:27Z","timestamp":1787598327750,"version":"build-2736575974"},"reference-count":87,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T00:00:00Z","timestamp":1659916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-14-CE-0018, ANR-10-LABX-62-IBEID"],"award-info":[{"award-number":["ANR-14-CE-0018, ANR-10-LABX-62-IBEID"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>The alternate growth of <jats:italic>Candida albicans<\/jats:italic> between a unicellular yeast form and a multicellular hyphal form is crucial for its ability to cause disease. Interestingly, both morphological forms support distinct functions during proliferation in the human host. We previously identified <jats:italic>ORF19.217<\/jats:italic> (C2_08890W_A), encoding a zinc-finger transcription factor of the C<jats:sub>2<\/jats:sub>H<jats:sub>2<\/jats:sub> family, in a systematic screen of genes whose overexpression contributes to <jats:italic>C. albicans<\/jats:italic>\u2019 morphological changes. Conditional overexpression of <jats:italic>ORF19.217<\/jats:italic> with the strong tetracycline-inducible promoter (P<jats:italic><jats:sub>TET<\/jats:sub><\/jats:italic>) resulted in a hyperfilamentous phenotype. We examined growth of the <jats:italic>orf19.217<\/jats:italic> knockout-mutant in different hypha-inducing conditions and found that the mutant still formed hyphae under standard hypha-inducing conditions. To further investigate the function of Orf19.217 in <jats:italic>C. albicans<\/jats:italic>, we combined genome-wide expression (RNA-Seq) and location (ChIP-Seq) analyses. We found that Orf19.217 is involved in regulatory processes comprising hyphal morphogenesis and iron acquisition. Comparative analysis with existing <jats:italic>C. albicans<\/jats:italic> hyphal transcriptomes indicates that Orf19.217-mediated filamentation is distinct from a true hyphal program. Further, the <jats:italic>orf19.217<\/jats:italic> knockout-mutant did not show increased sensitivity to iron deprivation, but <jats:italic>ORF19.217<\/jats:italic> overexpression was able to rescue the growth of a <jats:italic>hap5<\/jats:italic>-mutant, defective in a subunit of the CCAAT-complex, which is essential for iron acquisition. This suggested that Orf19.217 is involved in regulation of iron acquisition genes during iron deprivation and acts in a parallel pathway to the established CCAAT-complex. Interestingly, the <jats:italic>orf19.217<\/jats:italic>-mutant turned out to be defective in its ability to form filaments under iron-deficiency. Taken together our findings propose that the transcription factor Orf19.217 stimulates expression of the hyphal regulators <jats:italic>EFG1<\/jats:italic> and <jats:italic>BRG1<\/jats:italic> to promote filamentous growth under iron deprivation conditions, allowing the fungus to escape these iron-depleted conditions. The transcription factor therefore appears to be particularly important for adaptation of <jats:italic>C. albicans<\/jats:italic> to diverse environmental conditions in the human host. In regard to the newly identified functions, we have given the regulator the name Irf1, Iron-dependent Regulator of Filamentation.<\/jats:p>","DOI":"10.3389\/fcimb.2022.960884","type":"journal-article","created":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T06:04:30Z","timestamp":1659938670000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Functional Portrait of Irf1 (Orf19.217), a Regulator of Morphogenesis and Iron Homeostasis in Candida albicans"],"prefix":"10.3389","volume":"12","author":[{"given":"Lasse van","family":"Wijlick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadri","family":"Znaidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo","family":"Hern\u00e1ndez-Cervantes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Basso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Bachellier-Bassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"d\u2019Enfert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1128\/MMBR.00030-10","article-title":"Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers","volume":"75","author":"Abbas","year":"2011","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1000","DOI":"10.1111\/j.1567-1364.2009.00570.x","article-title":"Candida albicans iron acquisition within the host","volume":"9","author":"Almeida","year":"2009","journal-title":"FEMS Yeast Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"700","DOI":"10.12688\/f1000research.18546.1","article-title":"Recent advances in understanding Candida albicans hyphal growth","volume":"8","author":"Arkowitz","year":"2019","journal-title":"F1000Res"},{"key":"B4","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/82_2018_144","article-title":"From genes to networks: The regulatory circuitry controlling Candida albicans morphogenesis","volume":"422","author":"Basso","year":"2019","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"3648","DOI":"10.1128\/iai.61.9.3648-3655.1993","article-title":"Cloning and characterization of ECE1, a gene expressed in association with cell elongation of the dimorphic pathogen Candida albicans","volume":"61","author":"Birse","year":"1993","journal-title":"Infect. Immun."},{"key":"B6","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.chom.2011.08.001","article-title":"Candida albicans adds more weight to iron regulation","volume":"10","author":"Blankenship","year":"2011","journal-title":"Cell Host Microbe"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.3390\/jof3040057","article-title":"Global and multi-national prevalence of fungal diseases-estimate precision","volume":"3","author":"Bongomin","year":"2017","journal-title":"J. Fungi (Basel)"},{"key":"B8","doi-asserted-by":"publisher","first-page":"4753","DOI":"10.1093\/emboj\/20.17.4753","article-title":"NRG1, a repressor of filamentous growth in C. albicans, is down-regulated during filament induction","volume":"20","author":"Braun","year":"2001","journal-title":"EMBO J."},{"key":"B9","doi-asserted-by":"publisher","first-page":"165rv113","DOI":"10.1126\/scitranslmed.3004404","article-title":"Hidden killers: human fungal infections","volume":"4","author":"Brown","year":"2012","journal-title":"Sci. Transl. Med."},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004542","article-title":"Targeted changes of the cell wall proteome influence Candida albicans ability to form single- and multi-strain biofilms","volume":"10","author":"Cabral","year":"2014","journal-title":"PloS Pathog."},{"key":"B11","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1016\/j.chom.2013.04.010","article-title":"Iron in infection and immunity","volume":"13","author":"Cassat","year":"2013","journal-title":"Cell Host Microbe"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045912","article-title":"A versatile overexpression strategy in the pathogenic yeast Candida albicans: identification of regulators of morphogenesis and fitness","volume":"7","author":"Chauvel","year":"2012","journal-title":"PloS One"},{"key":"B13","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/j.chom.2011.07.005","article-title":"An iron homeostasis regulatory circuit with reciprocal roles in Candida albicans commensalism and pathogenesis","volume":"10","author":"Chen","year":"2011","journal-title":"Cell Host Microbe"},{"key":"B14","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1534\/genetics.111.127456","article-title":"The regulation of filamentous growth in yeast","volume":"190","author":"Cullen","year":"2012","journal-title":"Genetics"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00714-20","article-title":"Molecular elucidation of riboflavin production and regulation in Candida albicans, toward a novel antifungal drug target","volume":"5","author":"Demuyser","year":"2020","journal-title":"mSphere"},{"key":"B16","doi-asserted-by":"publisher","first-page":"1036","DOI":"10.1080\/21505594.2018.1482181","article-title":"Riboflavin and pantothenic acid biosynthesis are crucial for iron homeostasis and virulence in the pathogenic mold Aspergillus fumigatus","volume":"9","author":"Dietl","year":"2018","journal-title":"Virulence"},{"key":"B17","doi-asserted-by":"publisher","first-page":"1085","DOI":"10.1016\/j.jmb.2004.06.053","article-title":"Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans","volume":"341","author":"Echt","year":"2004","journal-title":"J. Mol. Biol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"2415","DOI":"10.1099\/00221287-145-9-2415","article-title":"A multicopper oxidase gene from Candida albicans: cloning, characterization and disruption","volume":"145","author":"Eck","year":"1999","journal-title":"Microbiol. (Reading)"},{"key":"B19","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/nrmicro.2015.21","article-title":"Interactions of fungal pathogens with phagocytes","volume":"14","author":"Erwig","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00185","article-title":"Iron at the centre of Candida albicans interactions","volume":"8","author":"Fourie","year":"2018","journal-title":"Front. Cell Infect. Microbiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1038\/nri3863","article-title":"Iron homeostasis in host defence and inflammation","volume":"15","author":"Ganz","year":"2015","journal-title":"Nat. Rev. Immunol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1068","DOI":"10.1016\/j.bbabio.2016.02.018","article-title":"The multitude of iron-sulfur clusters in respiratory complex I","volume":"1857","author":"Gnandt","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"B23","doi-asserted-by":"publisher","first-page":"W695","DOI":"10.1093\/nar\/gkq313","article-title":"A new bioinformatics analysis tools framework at EMBL-EBI","volume":"38","author":"Goujon","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"B24","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1007\/s00253-003-1335-2","article-title":"Molecular genetics of fungal siderophore biosynthesis and uptake: the role of siderophores in iron uptake and storage","volume":"62","author":"Haas","year":"2003","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1111\/j.1567-1364.2008.00394.x","article-title":"Iron deprivation induces EFG1-mediated hyphal development in Candida albicans without affecting biofilm formation","volume":"8","author":"Hameed","year":"2008","journal-title":"FEMS Yeast Res."},{"key":"B26","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1099\/00221287-146-4-869","article-title":"Candida albicans CFL1 encodes a functional ferric reductase activity that can rescue a Saccharomyces cerevisiae fre1 mutant","volume":"146","author":"Hammacott","year":"2000","journal-title":"Microbiol. (Reading)"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.770478","article-title":"Transcriptional control of hypoxic hyphal growth in the fungal pathogen Candida albicans","volume":"11","author":"Henry","year":"2021","journal-title":"Front. Cell Infect. Microbiol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"5246","DOI":"10.1128\/IAI.70.9.5246-5255.2002","article-title":"The siderophore iron transporter of Candida albicans (Sit1p\/Arn1p) mediates uptake of ferrichrome-type siderophores and is required for epithelial invasion","volume":"70","author":"Heymann","year":"2002","journal-title":"Infect. Immun."},{"key":"B29","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1039\/b906679a","article-title":"Chemistry and biology of siderophores","volume":"27","author":"Hider","year":"2010","journal-title":"Nat. Prod. Rep."},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000783","article-title":"A phenotypic profile of the Candida albicans regulatory network","volume":"5","author":"Homann","year":"2009","journal-title":"PloS Genet."},{"key":"B31","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1128\/EC.00158-10","article-title":"Candida albicans Hap43 is a repressor induced under low-iron conditions and is essential for iron-responsive transcriptional regulation and virulence","volume":"10","author":"Hsu","year":"2011","journal-title":"Eukaryot. Cell"},{"key":"B32","doi-asserted-by":"publisher","first-page":"30598","DOI":"10.1074\/jbc.M204545200","article-title":"Characterization and functional analysis of the siderophore-iron transporter CaArn1p in Candida albicans","volume":"277","author":"Hu","year":"2002","journal-title":"J. Biol. Chem."},{"key":"B33","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1586\/eri.11.152","article-title":"Candida albicans dimorphism as a therapeutic target","volume":"10","author":"Jacobsen","year":"2012","journal-title":"Expert Rev. Anti Infect. Ther."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1662","DOI":"10.1128\/EC.4.10.1662-1676.2005","article-title":"Novel regulatory function for the CCAAT-binding factor in Candida albicans","volume":"4","author":"Johnson","year":"2005","journal-title":"Eukaryot. Cell"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00779-21","article-title":"Bypass of Dfi1 regulation of Candida albicans invasive filamentation by iron limitation","volume":"7","author":"Junier","year":"2022","journal-title":"mSphere"},{"key":"B36","doi-asserted-by":"publisher","first-page":"2496","DOI":"10.1128\/MCB.21.7.2496-2505.2001","article-title":"Rfg1, a protein related to the Saccharomyces cerevisiae hypoxic regulator Rox1, controls filamentous growth and virulence in Candida albicans","volume":"21","author":"Kadosh","year":"2001","journal-title":"Mol. Cell Biol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"1894","DOI":"10.1128\/EC.00151-06","article-title":"Tcc1p, a novel protein containing the tetratricopeptide repeat motif, interacts with Tup1p to regulate morphological transition and virulence in Candida albicans","volume":"5","author":"Kaneko","year":"2006","journal-title":"Eukaryot. Cell"},{"key":"B38","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","article-title":"Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype","volume":"37","author":"Kim","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1099\/00221287-148-1-29","article-title":"Reductive iron uptake by Candida albicans: role of copper, iron and the TUP1 regulator","volume":"148","author":"Knight","year":"2002","journal-title":"Microbiol. (Reading)"},{"key":"B40","doi-asserted-by":"publisher","first-page":"5482","DOI":"10.1128\/IAI.73.9.5482-5492.2005","article-title":"Iron acquisition from transferrin by Candida albicans depends on the reductive pathway","volume":"73","author":"Knight","year":"2005","journal-title":"Infect. Immun."},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2020.118817","article-title":"Pathways of heme utilization in fungi","volume":"1867","author":"Kornitzer","year":"2020","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"B42","doi-asserted-by":"publisher","first-page":"1185","DOI":"10.1046\/j.1365-2958.2003.03368.x","article-title":"Molecular mechanisms of iron uptake in fungi","volume":"47","author":"Kosman","year":"2003","journal-title":"Mol. Microbiol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/S0968-0004(03)00025-2","article-title":"An eight-cysteine-containing CFEM domain unique to a group of fungal membrane proteins","volume":"28","author":"Kulkarni","year":"2003","journal-title":"Trends Biochem. Sci."},{"key":"B44","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1146\/annurev.micro.59.030804.121034","article-title":"Alternative Candida albicans lifestyles: growth on surfaces","volume":"59","author":"Kumamoto","year":"2005","journal-title":"Annu. Rev. Microbiol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/BF00330941","article-title":"Isolation of hem3 mutants from Candida albicans by sequential gene disruption","volume":"217","author":"Kurtz","year":"1989","journal-title":"Mol. Gen. Genet."},{"key":"B46","doi-asserted-by":"publisher","first-page":"e1004407","DOI":"10.1371\/journal.ppat.1004407","article-title":"A relay network of extracellular heme-binding proteins drives C. albicans iron acquisition from hemoglobin","volume":"10","author":"Kuznets","year":"2014","journal-title":"PloS Pathog."},{"key":"B47","doi-asserted-by":"publisher","first-page":"1451","DOI":"10.1111\/j.1365-2958.2004.04214.x","article-title":"Regulatory networks affected by iron availability in Candida albicans","volume":"53","author":"Lan","year":"2004","journal-title":"Mol. Microbiol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"6935","DOI":"10.1093\/nar\/gky594","article-title":"Generating genomic platforms to study Candida albicans pathogenesis","volume":"46","author":"Legrand","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"B49","doi-asserted-by":"publisher","first-page":"1679","DOI":"10.1534\/genetics.116.190645","article-title":"Systematic genetic screen for transcriptional regulators of the Candida albicans white-opaque switch","volume":"203","author":"Lohse","year":"2016","journal-title":"Genetics"},{"key":"B50","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.1128\/EC.3.5.1076-1087.2004","article-title":"Transcriptional response of Candida albicans upon internalization by macrophages","volume":"3","author":"Lorenz","year":"2004","journal-title":"Eukaryot. Cell"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"B52","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.bbrc.2021.05.039","article-title":"Candida albicans requires iron to sustain hyphal growth","volume":"561","author":"Luo","year":"2021","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"B53","doi-asserted-by":"publisher","first-page":"1428","DOI":"10.1038\/nprot.2014.083","article-title":"Motif-based analysis of large nucleotide data sets using MEME-ChIP","volume":"9","author":"Ma","year":"2014","journal-title":"Nat. Protoc."},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058613","article-title":"A core filamentation response network in Candida albicans is restricted to eight genes","volume":"8","author":"Martin","year":"2013","journal-title":"PloS One"},{"key":"B55","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1002\/yea.3031","article-title":"bPeaks: a bioinformatics tool to detect transcription factor binding sites from ChIPseq data in yeasts and other organisms with small genomes","volume":"31","author":"Merhej","year":"2014","journal-title":"Yeast"},{"key":"B56","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1002\/pro.5560070102","article-title":"Covalent attachment of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) to enzymes: the current state of affairs","volume":"7","author":"Mewies","year":"1998","journal-title":"Protein Sci."},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00130-16","article-title":"Candida albicans gene deletion with a transient CRISPR-Cas9 system","volume":"1","author":"Min","year":"2016","journal-title":"mSphere"},{"key":"B58","doi-asserted-by":"publisher","first-page":"D642","DOI":"10.1093\/nar\/gkz859","article-title":"YEASTRACT+: a portal for cross-species comparative genomics of transcription regulation in yeasts","volume":"48","author":"Monteiro","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"B59","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1038\/nature17625","article-title":"Candidalysin is a fungal peptide toxin critical for mucosal infection","volume":"532","author":"Moyes","year":"2016","journal-title":"Nature"},{"key":"B60","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1186\/s40168-017-0373-4","article-title":"The gut mycobiome of the human microbiome project healthy cohort","volume":"5","author":"Nash","year":"2017","journal-title":"Microbiome"},{"key":"B61","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1016\/j.mib.2013.09.006","article-title":"Candida albicans specializations for iron homeostasis: from commensalism to virulence","volume":"16","author":"Noble","year":"2013","journal-title":"Curr. Opin. Microbiol."},{"key":"B62","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1128\/EC.4.2.298-309.2005","article-title":"Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans","volume":"4","author":"Noble","year":"2005","journal-title":"Eukaryot. Cell"},{"key":"B63","doi-asserted-by":"publisher","first-page":"1088","DOI":"10.1038\/ng.2710","article-title":"Passage through the mammalian gut triggers a phenotypic switch that promotes Candida albicans commensalism","volume":"45","author":"Pande","year":"2013","journal-title":"Nat. Genet."},{"key":"B64","doi-asserted-by":"publisher","first-page":"1328","DOI":"10.1128\/EC.4.8.1328-1342.2005","article-title":"Tetracycline-inducible gene expression and gene deletion in Candida albicans","volume":"4","author":"Park","year":"2005","journal-title":"Eukaryot. Cell"},{"key":"B65","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1002\/yea.1539","article-title":"Expression of Candida albicans Sfu1 in fission yeast complements the loss of the iron-regulatory transcription factor Fep1 and requires tup co-repressors","volume":"24","author":"Pelletier","year":"2007","journal-title":"Yeast"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001510","article-title":"Candida albicans commensalism and pathogenicity are intertwined traits directed by a tightly knit transcriptional regulatory circuit","volume":"11","author":"Perez","year":"2013","journal-title":"PloS Biol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/0092-8674(89)90903-3","article-title":"Functional dissection and sequence of yeast HAP1 activator","volume":"56","author":"Pfeifer","year":"1989","journal-title":"Cell"},{"key":"B68","doi-asserted-by":"publisher","first-page":"e00117","DOI":"10.1128\/mBio.00117-12","article-title":"Variation in Candida albicans EFG1 expression enables host-dependent changes in colonizing fungal populations","volume":"3","author":"Pierce","year":"2012","journal-title":"mBio"},{"key":"B69","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1093\/bioinformatics\/btu627","article-title":"FungiFun2: a comprehensive online resource for systematic analysis of gene lists from fungal species","volume":"31","author":"Priebe","year":"2015","journal-title":"Bioinformatics"},{"key":"B70","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1111\/mmi.14818","article-title":"Overexpression approaches to advance understanding of Candida albicans","volume":"117","author":"Rai","year":"2022","journal-title":"Mol. Microbiol."},{"key":"B71","doi-asserted-by":"publisher","first-page":"1062","DOI":"10.1126\/science.288.5468.1062","article-title":"A high-affinity iron permease essential for Candida albicans virulence","volume":"288","author":"Ramanan","year":"2000","journal-title":"Science"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.605711","article-title":"Transcriptional circuits regulating developmental processes in Candida albicans","volume":"10","author":"Rodriguez","year":"2020","journal-title":"Front. Cell Infect. Microbiol."},{"key":"B73","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1128\/EC.00034-10","article-title":"Adaptations of Candida albicans for growth in the mammalian intestinal tract","volume":"9","author":"Rosenbach","year":"2010","journal-title":"Eukaryot. Cell"},{"key":"B74","doi-asserted-by":"publisher","first-page":"2300","DOI":"10.1128\/AAC.40.10.2300","article-title":"Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors","volume":"40","author":"Sanglard","year":"1996","journal-title":"Antimicrob. Agents Chemother."},{"key":"B75","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: a software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"B76","doi-asserted-by":"publisher","first-page":"25154","DOI":"10.1074\/jbc.M111.233569","article-title":"Cap2-HAP complex is a critical transcriptional regulator that has dual but contrasting roles in regulation of iron homeostasis in Candida albicans","volume":"286","author":"Singh","year":"2011","journal-title":"J. Biol. Chem."},{"key":"B77","doi-asserted-by":"publisher","first-page":"1982","DOI":"10.1093\/emboj\/16.8.1982","article-title":"Efg1p, an essential regulator of morphogenesis of the human pathogen Candida albicans, is a member of a conserved class of bHLH proteins regulating morphogenetic processes in fungi","volume":"16","author":"Stoldt","year":"1997","journal-title":"EMBO J."},{"key":"B78","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/978-3-540-70618-2_6","article-title":"Polarised growth in fungi","volume-title":"Biology of the fungal cell","author":"Sudbery","year":"2007"},{"key":"B79","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.tim.2004.05.008","article-title":"The distinct morphogenic states of Candida albicans","volume":"12","author":"Sudbery","year":"2004","journal-title":"Trends Microbiol."},{"key":"B80","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1093\/bib\/bbs017","article-title":"Integrative genomics viewer (IGV): high-performance genomics data visualization and exploration","volume":"14","author":"Thorvaldsdottir","year":"2013","journal-title":"Brief Bioinform."},{"key":"B81","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1500248","article-title":"A Candida albicans CRISPR system permits genetic engineering of essential genes and gene families","volume":"1","author":"Vyas","year":"2015","journal-title":"Sci. Adv."},{"key":"B82","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1001\/jama.231.1.39","article-title":"Nutritional immunity. host's attempt to withold iron from microbial invaders","volume":"231","author":"Weinberg","year":"1975","journal-title":"JAMA"},{"key":"B83","doi-asserted-by":"publisher","first-page":"1551","DOI":"10.1046\/j.1365-2958.2002.02976.x","article-title":"Deletion of the copper transporter CaCCC2 reveals two distinct pathways for iron acquisition in Candida albicans","volume":"44","author":"Weissman","year":"2002","journal-title":"Mol. Microbiol."},{"key":"B84","doi-asserted-by":"publisher","first-page":"1002","DOI":"10.1016\/j.chom.2021.03.019","article-title":"Recording of DNA-binding events reveals the importance of a repurposed Candida albicans regulatory network for gut commensalism","volume":"29","author":"Witchley","year":"2021","journal-title":"Cell Host Microbe"},{"key":"B85","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1016\/j.chom.2019.02.008","article-title":"Candida albicans morphogenesis programs control the balance between gut commensalism and invasive infection","volume":"25","author":"Witchley","year":"2019","journal-title":"Cell Host Microbe"},{"key":"B86","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1111\/j.1365-2958.2011.07704.x","article-title":"Functional characterization of the ferroxidase, permease high-affinity iron transport complex from Candida albicans","volume":"81","author":"Ziegler","year":"2011","journal-title":"Mol. Microbiol."},{"key":"B87","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12890","article-title":"Systematic gene overexpression in Candida albicans identifies a regulator of early adaptation to the mammalian gut","volume":"20","author":"Znaidi","year":"2018","journal-title":"Cell Microbiol."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2022.960884\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T06:04:36Z","timestamp":1659938676000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2022.960884\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,8]]},"references-count":87,"alternative-id":["10.3389\/fcimb.2022.960884"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2022.960884","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,8]]},"article-number":"960884"}}