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Cell. Infect. Microbiol."],"abstract":"<jats:p>Clonorchiasis caused by <jats:italic>Clonorchis sinensis<\/jats:italic> is a mainly foodborne parasitic disease. It can lead to hepatobiliary duct inflammation, fibrosis, obstructive jaundice, liver cirrhosis, and even cholangiocarcinoma. Interleukin (IL)-10 is an immune-regulatory cytokine which plays an immunosuppressive role during infection. Our previous study found that IL-10 was increased in mice with <jats:italic>C. sinensis<\/jats:italic> infection. However, the role and mechanism of IL-10 playing in hepatobiliary injury induced by <jats:italic>C. sinensis<\/jats:italic> infection remain unknown. Herein, <jats:italic>Il10<jats:sup>+\/+<\/jats:sup><\/jats:italic> mice and <jats:italic>Il10<jats:sup>+\/-<\/jats:sup><\/jats:italic> C57BL\/6J mice were infected with <jats:italic>C. sinensis<\/jats:italic>. It was found that IL-10 deficiency aggravated biliary hyperplasia and exacerbated periductal fibrosis induced by <jats:italic>C. sinensis<\/jats:italic> infection. Moreover, IL-10 deficiency increased CD4<jats:sup>+<\/jats:sup>T cells and CD8<jats:sup>+<\/jats:sup>T cells but not macrophages in the liver of mice with infection. There were no apparent differences in Th1 and Treg cells between <jats:italic>Il10<jats:sup>+\/+<\/jats:sup><\/jats:italic> and <jats:italic>Il10<jats:sup>+\/-<\/jats:sup><\/jats:italic> mice infected with <jats:italic>C. sinensis<\/jats:italic>. However, the proportion of Th17 cells in CD4<jats:sup>+<\/jats:sup>T cells in <jats:italic>Il10<jats:sup>+\/-<\/jats:sup><\/jats:italic> infected mice was significantly higher than that in <jats:italic>Il10<jats:sup>+\/+<\/jats:sup><\/jats:italic> infected mice. IL-10 deficiency also enhanced the increase of Th17 cells induced by ESPs stimulation <jats:italic>in vitro<\/jats:italic>. Taken together, our results suggest that IL-10 plays a protective role in hepatobiliary injury in C57BL\/6J mice induced by <jats:italic>C. sinensis<\/jats:italic> infection <jats:italic>via<\/jats:italic> inhibiting Th17 cells, which could deepen our understanding of the immunopathology of clonorchiasis.<\/jats:p>","DOI":"10.3389\/fcimb.2022.994838","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T06:16:52Z","timestamp":1665641812000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["IL-10 regulates Th17 response to inhibit hepatobiliary injury caused by Clonorchis sinensis infection in C57BL\/6J mice"],"prefix":"10.3389","volume":"12","author":[{"given":"Beibei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianling","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianqian","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guozhi","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Hua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinyao","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephane","family":"Koda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin-Hai","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihua","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kui-Yang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1016\/j.pt.2019.08.006","article-title":"Understanding CD8(+) T cell immunity to trypanosoma cruzi and how to improve it","volume":"35","author":"Acosta Rodriguez","year":"2019","journal-title":"Trends Parasitol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1016\/j.jaci.2016.06.033","article-title":"Interleukins (from IL-1 to IL-38), interferons, transforming growth factor beta, and TNF-alpha: Receptors, functions, and roles in diseases","volume":"138","author":"Akdis","year":"2016","journal-title":"J. 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