{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:18:45Z","timestamp":1791919125615,"version":"4.3.4"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T00:00:00Z","timestamp":1677542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Sexually transmitted chlamydia and gonorrhea infections caused by the bacteria <jats:italic>Chlamydia trachomatis<\/jats:italic> and <jats:italic>Neisseria gonorrhoeae<\/jats:italic> remain a major public health concern worldwide, particularly in less developed nations. It is crucial to use a point of care (POC) diagnostic method that is quick, specific, sensitive, and user-friendly to treat and control these infections effectively. Here, a novel molecular diagnostic assay, combining multiplex loop-mediated isothermal amplification (mLAMP) with a visual gold nanoparticles-based lateral flow biosensor (AuNPs-LFB) was devised and used for highly specific, sensitive, rapid, visual, and easy identification of <jats:italic>C. trachomatis<\/jats:italic> and <jats:italic>N. gonorrhoeae<\/jats:italic>. Two unique independent primer pairs were successful designed against the <jats:italic>ompA<\/jats:italic> and <jats:italic>orf1<\/jats:italic> genes of <jats:italic>C. trachomatis<\/jats:italic> and <jats:italic>N. gonorrhoeae<\/jats:italic>, respectively. The optimal mLAMP-AuNPs-LFB reaction conditions were determined to be 67\u00b0C for 35\u00a0min. The detection procedure, involving crude genomic DNA extraction (~5 min), LAMP amplification (35\u00a0min), and visual results interpretation (&amp;lt;2\u00a0min), can be completed within 45\u00a0min. Our assay has a detection limit of 50 copies per test, and we did not observe any cross-reactivity with any other bacteria in our testing. Hence, our mLAMP-AuNPs-LFB assay can potentially be used for POC testing to detect <jats:italic>C. trachomatis<\/jats:italic> and <jats:italic>N. gonorrhoeae<\/jats:italic> in clinical settings, particularly in underdeveloped regions.<\/jats:p>","DOI":"10.3389\/fcimb.2023.1067554","type":"journal-article","created":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T16:57:26Z","timestamp":1678121846000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":28,"title":["Visual and rapid identification of Chlamydia trachomatis and Neisseria gonorrhoeae using multiplex loop-mediated isothermal amplification and a gold nanoparticle-based lateral flow biosensor"],"prefix":"10.3389","volume":"13","author":[{"given":"Xu","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingxue","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanfang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilei","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,2,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00211-21","article-title":"Diagnostic tests for detecting Chlamydia trachomatis and Neisseria gonorrhoeae in rectal and pharyngeal specimens","volume":"60","author":"Adamson","year":"2022","journal-title":"J. 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