{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T15:24:17Z","timestamp":1781709857037,"version":"3.54.5"},"reference-count":62,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T00:00:00Z","timestamp":1674172800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Ixodes rici nus<\/jats:italic> and <jats:italic>Ixodes persulcatus<\/jats:italic> ticks are the main vectors of tick-borne encephalitis virus (TBEV), which has three main subtypes connected with certain tick species: the European subtype, associated with <jats:italic>I. ricinus<\/jats:italic>, and the Siberian and Far-Eastern subtypes, associated with <jats:italic>I. persulcatus<\/jats:italic>. Distribution ranges of these species overlap and form large sympatric areas in the East European Plain and Baltic countries. It has previously been shown that crossing of <jats:italic>I. ricinus<\/jats:italic> and <jats:italic>I. persulcatus<\/jats:italic> is possible, with the appearance of sterile hybrids. Hybridization of ticks can affect not only the spread of ticks but also the properties of natural foci of arbovirus infections, in particular TBEV. In the present study, we analyzed the effectiveness of virus transmission from infected mice to larvae and nymphs and trans-stadial transmission (from larvae to nymph and adult) in <jats:italic>I. ricinus<\/jats:italic>, <jats:italic>I. persulcatus<\/jats:italic>, and hybrids. For this purpose, we bred a hybrid generation from the crossing of <jats:italic>I. persulcatus<\/jats:italic> females and <jats:italic>I. ricinus<\/jats:italic> males, and we used the Siberian and European subtypes of TBEV. We showed that after feeding on infected mice, virus prevalence in engorged ticks decreased over time, and after molting, the opposite was true. In hybrids we observed the highest acquisition effectiveness and RNA copy numbers during Siberian TBEV subtype transmission. The efficiency of trans-stadial transmission of both TBEV subtypes was similar in hybrids and parental species. After the second trans-stadial TBEV transmission, a significant increase in ticks\u2019 infection rates was observed only in specific subtype-tick combination. Our data demonstrate the possible features of TBEV circulation in the <jats:italic>I. ricinus<\/jats:italic> and <jats:italic>I. persulcatus<\/jats:italic> sympatry area.<\/jats:p>","DOI":"10.3389\/fcimb.2023.1104484","type":"journal-article","created":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T05:52:46Z","timestamp":1674193966000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Hybrids of Ixodes ricinus and Ixodes persulcatus ticks effectively acquire and transmit tick-borne encephalitis virus"],"prefix":"10.3389","volume":"13","author":[{"given":"Oxana A.","family":"Belova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra E.","family":"Polienko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia D.","family":"Averianova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina G.","family":"Karganova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,1,20]]},"reference":[{"key":"B1","first-page":"48","article-title":"The exchange of the tick-borne encephalitis virus between ixodid ticks feeding jointly on animals with a subthreshold level of viremia","volume":"2","author":"Alekseev","year":"1990","journal-title":"Med. 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