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Cell. Infect. Microbiol."],"abstract":"<jats:p>The conventional treatment of congenital toxoplasmosis is mainly based on the combination of sulfadiazine and pyrimethamine. However, therapy with these drugs is associated with severe side effects and resistance, requiring the study of new therapeutic strategies. There are currently many studies with natural products, including <jats:italic>Copaifera<\/jats:italic> oleoresin, showing actions against some pathogens, as <jats:italic>Trypanosoma cruzi<\/jats:italic> and <jats:italic>Leishmania<\/jats:italic>. In the present study, we investigated the effects of the leaf hydroalcoholic extract and oleoresin from <jats:italic>Copaifera multijuga<\/jats:italic> against <jats:italic>Toxoplasma gondii<\/jats:italic> in human villous (BeWo) and extravillous (HTR8\/SVneo) trophoblast cells, as well as in human villous explants from third-trimester pregnancy. For this purpose, both cells and villous explants were infected or not with <jats:italic>T. gondii<\/jats:italic>, treated with hydroalcoholic extract or oleoresin from <jats:italic>C. multijuga<\/jats:italic> and analyzed for toxicity, parasite proliferation, cytokine and ROS production. In parallel, both cells were infected by tachyzoites pretreated with hydroalcoholic extract or oleoresin, and adhesion, invasion and replication of the parasite were observed. Our results showed that the extract and oleoresin did not trigger toxicity in small concentrations and were able to reduce the <jats:italic>T. gondii<\/jats:italic> intracellular proliferation in cells previously infected. Also, the hydroalcoholic extract and oleoresin demonstrated an irreversible antiparasitic action in BeWo and HTR8\/SVneo cells. Next, adhesion, invasion and replication of <jats:italic>T. gondii<\/jats:italic> were dampened when BeWo or HTR8\/SVneo cells were infected with pretreated tachyzoites. Finally, infected and treated BeWo cells upregulated IL-6 and downmodulated IL-8, while HTR8\/SVneo cells did not change significantly these cytokines when infected and treated. Finally, both the extract and oleoresin reduced the <jats:italic>T. gondii<\/jats:italic> proliferation in human explants, and no significant changes were observed in relation to cytokine production. Thus, compounds from <jats:italic>C. multijuga<\/jats:italic> presented different antiparasitic activities that were dependent on the experimental model, being the direct action on tachyzoites a common mechanism operating in both cells and villi. Considering all these parameters, the hydroalcoholic extract and oleoresin from <jats:italic>C. multijuga<\/jats:italic> can be a target for the establishment of new therapeutic strategy for congenital toxoplasmosis.<\/jats:p>","DOI":"10.3389\/fcimb.2023.1113896","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T05:54:13Z","timestamp":1676267653000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Leaf hydroalcoholic extract and oleoresin from Copaifera multijuga control Toxoplasma gondii infection in human trophoblast cells and placental explants from third-trimester pregnancy"],"prefix":"10.3389","volume":"13","author":[{"given":"Aryani Felixa Fajardo","family":"Mart\u00ednez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel Cota","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra Monteiro","family":"Rosini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joed Pires de Lima","family":"J\u00fanior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Nogueira","family":"Melo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelvin Orlando Espinoza","family":"Bland\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelica Oliveira","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio Ricardo","family":"Ambr\u00f3sio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Cassio Sola","family":"Veneziani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jairo Kenupp","family":"Bastos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Henrique Gomes","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eloisa Am\u00e1lia Vieira","family":"Ferro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bellisa Freitas","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,2,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"688","DOI":"10.1186\/s13104-019-4732-z","article-title":"Anti-Toxoplasma activity of sorghum bicolor-derived lipophilic fractions","volume":"12","author":"Abugri","year":"2019","journal-title":"BMC Res. 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