{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T17:50:42Z","timestamp":1788630642363,"version":"build-2803163510"},"reference-count":14,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T00:00:00Z","timestamp":1685404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Klebsiella pneumoniae<\/jats:italic> is a global threat to healthcare, and despite the availability of new drugs, polymyxins are still an important therapeutic option for this and other resistant gram-negative pathogens. Broth microdilution is the only method that is recommended for polymyxins. In this study, we evaluated the accuracy of a commercial Policimbac\u00ae plate in determining the polymyxin B MIC for <jats:italic>K. pneumoniae<\/jats:italic> clinical isolates. The results were compared with those of the broth microdilution method according to ISO 16782. The Policimbac\u00ae plate had an excellent 98.04% categorical agreement, but unacceptable 31.37% essential agreement rates. Almost 2% of major errors as observed. Additionally, 52.94% of the strains overestimated the MIC at 1 \u00b5g\/mL. Three isolates were excluded from the analysis due to the drying of the Policimbac\u00ae plate. To avoid dryness, we included wet gauze for the test, obtaining a 100% of categorical agreement rate; however, a low essential agreement was maintained (25.49%). In conclusion, the Policimbac\u00ae plate was unable to correctly determine the polymyxin B MIC for <jats:italic>K. pneumoniae<\/jats:italic> isolates. This low performance may interfere with the clinical use of the drug and, thus, with the result of the patient\u2019s treatment.<\/jats:p>","DOI":"10.3389\/fcimb.2023.1139784","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T13:09:05Z","timestamp":1685538545000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Low performance of Policimbac\u00ae broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae"],"prefix":"10.3389","volume":"13","author":[{"given":"Nat\u00e1lia Concei\u00e7\u00e3o","family":"Rocha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica Mariana","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolayne Larissa","family":"Russi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jussara Kasuko","family":"Palmeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Girardello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,5,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2023.115930","article-title":"Dissemination of NDM-producing bacteria in southern Brazil","volume":"106","author":"Arend","year":"2023","journal-title":"Diagn. 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