{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T15:28:27Z","timestamp":1772033307577,"version":"3.50.1"},"reference-count":20,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T00:00:00Z","timestamp":1688342400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012245","name":"Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"publisher","award":["2020A0505100039"],"award-info":[{"award-number":["2020A0505100039"]}],"id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004000","name":"Guangzhou Science and Technology Program key projects","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004000","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Previous studies have indicated that nitric oxide synthase 2 (<jats:italic>NOS2<\/jats:italic>) genetic variations are involved in delayed fracture healing and fracture non-union. Whether these genetic variants associate with the development of osteomyelitis (OM) remains unclear. Here, we analyzed the potential relationships between <jats:italic>NOS2<\/jats:italic> genetic variations and the risk of developing post-traumatic OM (PTOM) in a Chinese Han population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Altogether 704 participants, including 336 PTOM patients and 368 healthy controls, were genotyped of rs2297514 and rs2248814 of the <jats:italic>NOS2<\/jats:italic> gene using the SNaPshot genotyping method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Outcomes showed that the frequency of allele C of rs2297514 in the patient group was significantly lower than that in the control group (48.7% <jats:italic>vs<\/jats:italic>. 54.5%, <jats:italic>P<\/jats:italic> = 0.029, OR = 0.792, 95% CI 0.642 \u2013 0.976). In addition, significant associations were found between rs2297514 and susceptibility to PTOM by the recessive model (<jats:italic>P<\/jats:italic> = 0.007, OR = 0.633, 95% CI 0.453 \u2013 0.884), and the homozygous model (<jats:italic>P<\/jats:italic> = 0.039, OR = 0.648, 95% CI 0.429 \u2013 0.979). Moreover, patients with the CC genotype of rs2297514 had lower inflammatory biomarkers levels than the TT genotype, especially for the C-reactive protein (CRP) level (median: 4.1 mg\/L <jats:italic>vs<\/jats:italic>. 8.9 mg\/L, <jats:italic>P<\/jats:italic> = 0.027). However, no significant relationship was noted between rs2248814 and the risk of developing PTOM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this Chinese cohort, rs2297514 is correlated with a decreased risk of PTOM development, with genotype CC as a protective factor.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2023.1177830","type":"journal-article","created":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T05:34:11Z","timestamp":1688362451000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Nitric oxide synthase 2 genetic variation rs2297514 associates with a decreased susceptibility to extremity post-traumatic osteomyelitis in a Chinese Han population"],"prefix":"10.3389","volume":"13","author":[{"given":"Chen-sheng","family":"Song","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-rong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-yu","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-qiu","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan-jun","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,7,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"556","DOI":"10.1080\/17453674.2017.1348439","article-title":"A link between osteomyelitis and IL1RN and IL1B polymorphisms-a study in patients from northeast Brazil","volume":"88","author":"Alves De Souza","year":"2017","journal-title":"Acta orthop"},{"key":"B2","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1097\/QCO.0000000000000155","article-title":"Current concepts in pathogenesis of acute and chronic osteomyelitis","volume":"28","author":"Beck-Broichsitter","year":"2015","journal-title":"Curr. 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