{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T14:44:46Z","timestamp":1792853086772,"version":"4.5.0"},"reference-count":64,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010076","name":"Conseil R\u00e9gional Provence-Alpes-C\u00f4te d'Azur","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010076","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Burkholderia thailandensis<\/jats:italic> is a study model for <jats:italic>Burkholderia pseudomallei<\/jats:italic>, a highly virulent pathogen, known to be the causative agent of melioidosis and a potential bioterrorism agent. These two bacteria use an (acyl-homoserine lactone) AHL-mediated quorum sensing (QS) system to regulate different behaviors including biofilm formation, secondary metabolite productions, and motility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using an enzyme-based quorum quenching (QQ) strategy, with the lactonase <jats:italic>Sso<\/jats:italic>Pox having the best activity on <jats:italic>B. thailandensis<\/jats:italic> AHLs, we evaluated the importance of QS in <jats:italic>B. thailandensis<\/jats:italic> by combining proteomic and phenotypic analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We demonstrated that QS disruption largely affects overall bacterial behavior including motility, proteolytic activity, and antimicrobial molecule production. We further showed that QQ treatment drastically decreases <jats:italic>B. thailandensis<\/jats:italic> bactericidal activity against two bacteria (<jats:italic>Chromobacterium violaceum<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic>), while a spectacular increase in antifungal activity was observed against fungi and yeast (<jats:italic>Aspergillus niger<\/jats:italic>, <jats:italic>Fusarium graminearum<\/jats:italic> and <jats:italic>Saccharomyces cerevisiae<\/jats:italic>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>This study provides evidence that QS is of prime interest when it comes to understanding the virulence of <jats:italic>Burkholderia<\/jats:italic> species and developing alternative treatments.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2023.1190859","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T10:35:15Z","timestamp":1685702115000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Lactonase-mediated inhibition of quorum sensing largely alters phenotypes, proteome, and antimicrobial activities in Burkholderia thailandensis E264"],"prefix":"10.3389","volume":"13","author":[{"given":"M\u00e9lanie","family":"Gonzales","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Plener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Armengaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9ric","family":"Chabri\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Daud\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,6,2]]},"reference":[{"key":"B1","unstructured":"AdlerD.\n            KellyT. 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