{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:20:44Z","timestamp":1791840044158,"version":"4.3.4"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T00:00:00Z","timestamp":1692921600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>According to WHO, antibiotic resistance is increasing to hazardous levels worldwide. Candidiasis often occurs after taking antibiotics. Therefore, antibiotic resistance is a global problem and searching for antibacterial agents is necessary.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To determine the antimicrobial activity of bacterial lysate of <jats:italic>Lactobacillus (L.) rhamnosus<\/jats:italic> DV separately and with plant extracts against bacterial and yeast test cultures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Antimicrobial activity of Del-Immune V<jats:sup>\u00ae<\/jats:sup> (cell wall and DNA fragments from a <jats:italic>L. rhamnosus<\/jats:italic> DV) separately and with cinnamon, beetroot, and blackcurrant extracts was determined by the minimum inhibitory concentration (MIC). Twofold serial dilutions determined the MIC in previously prepared meat-peptone broth (MPB) for bacteria and liquid wort for yeast. In the study, gram-negative (<jats:italic>Escherichia coli<\/jats:italic> IEM-1, <jats:italic>Proteus vulgaris<\/jats:italic> P\u0410-12, <jats:italic>Pseudomonas<\/jats:italic> sp. MI-2, <jats:italic>L. rhamnosus<\/jats:italic> 13\/2) and gram-positive (<jats:italic>Bacillus (B.) subtilis<\/jats:italic> B\u0422-2, <jats:italic>Staphylococcus aureus<\/jats:italic> B\u041c\u0421-1) bacteria, as well as yeast (<jats:italic>Candida (C.) albicans<\/jats:italic> D-6, <jats:italic>C. tropicalis<\/jats:italic> PE-2, <jats:italic>C. utilis<\/jats:italic> BVS-65) were used as test cultures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The MIC for the studied bacterial test cultures after application of <jats:italic>L. rhamnosus<\/jats:italic> DV bacterial lysates was from 1.0 \u00b1 0.05 mg\/mL to 12.5 \u00b1 0.63 mg\/mL, which was significantly less than that of the thermally inactivated control (MIC from 125.0 \u00b1 6.25 mg\/mL to 250.0 \u00b1 12.5 mg\/mL). <jats:italic>B. subtilis<\/jats:italic> BT-2 culture was the least sensitive to the action of the bacterial lysate (MIC\u201412.5 \u00b1 0.63 mg\/mL). It showed the best antibacterial and antifungal effect bacterial lysate with the phytonutrient blackcurrant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>It was demonstrated that bacterial lysate of lactic acid bacteria <jats:italic>L. rhamnosus<\/jats:italic> DV exhibits antibacterial and antifungal properties during direct contact with pathogenic agents.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2023.1211952","type":"journal-article","created":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T17:49:34Z","timestamp":1692985774000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Antimicrobial activity of dietary supplements based on bacterial lysate of Lactobacillus rhamnosus DV"],"prefix":"10.3389","volume":"13","author":[{"given":"Yurii","family":"Penchuk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryana","family":"Savytska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazarii","family":"Kobyliak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danylo","family":"Ostapchenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Kolodiy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svitlana","family":"Onysenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olena","family":"Tsyryuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleksandr","family":"Korotkyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fedir","family":"Grygoriev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetyana","family":"Falalyeyeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,8,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/S0168-1605(02)00169-1","article-title":"Antibacterial activity of extracts from some edible plants commonly consumed in Asia","volume":"80","author":"Alzoreky","year":"2003","journal-title":"Int. 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