{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:46:56Z","timestamp":1790725616274,"version":"4.1.0"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T00:00:00Z","timestamp":1694736000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    The surge of multidrug-resistant fungal pathogens, especially\n                    <jats:italic>Candida auris<\/jats:italic>\n                    , poses significant threats to global public health.\n                    <jats:italic>Candida auris<\/jats:italic>\n                    exhibits resistance to multiple antifungal drugs, leading to major outbreaks and a high mortality rate. With an urgent call for innovative therapeutic strategies, this study focused on the regulation and pathobiological significance of secreted aspartyl proteinases (SAPs) in\n                    <jats:italic>C. auris<\/jats:italic>\n                    , as these enzymes play pivotal roles in the virulence of some fungal species. We delved into the Ras\/cAMP\/PKA signaling pathway\u2019s influence on SAP activity in\n                    <jats:italic>C. auris<\/jats:italic>\n                    . Our findings underscored that the Ras\/cAMP\/PKA pathway significantly modulates SAP activity, with PKA catalytic subunits, Tpk1 and Tpk2, playing a key role. We identified a divergence in the SAPs of\n                    <jats:italic>C. auris<\/jats:italic>\n                    compared to\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , emphasizing the variation between\n                    <jats:italic>Candida<\/jats:italic>\n                    species. Among seven identified secreted aspartyl proteases in\n                    <jats:italic>C. auris<\/jats:italic>\n                    (Sapa1 to Sapa7), Sapa3 emerged as the primary SAP in the pathogen. Deletion of Sapa3 led to a significant decline in SAP activity. Furthermore, we have established the involvement of Sapa3 in the biofilm formation of\n                    <jats:italic>C. auris<\/jats:italic>\n                    . Notably, Sapa3 was primarily regulated by Tpk1 and Tpk2. Deletion of\n                    <jats:italic>SAPA3<\/jats:italic>\n                    significantly reduced\n                    <jats:italic>C. auris<\/jats:italic>\n                    virulence, underscoring its pivotal role in\n                    <jats:italic>C. auris<\/jats:italic>\n                    pathogenicity. The outcomes of this study provide valuable insights into potential therapeutic targets, laying the groundwork for future interventions against\n                    <jats:italic>C. auris<\/jats:italic>\n                    infection.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2023.1257897","type":"journal-article","created":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T01:45:45Z","timestamp":1695001545000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":39,"title":["Secreted aspartyl protease 3 regulated by the Ras\/cAMP\/PKA pathway promotes the virulence of Candida auris"],"prefix":"10.3389","volume":"13","author":[{"given":"Ji-Seok","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Tae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Sun","family":"Bahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,9,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"807","DOI":"10.3390\/microorganisms9040807","article-title":"Candida auris: epidemiology, diagnosis, pathogenesis, antifungal susceptibility, and infection control measures to combat the spread of infections in healthcare facilities","volume":"9","author":"Ahmad","year":"2021","journal-title":"Microorganisms"},{"key":"B2","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1007\/s12281-023-00451-7","article-title":"Strategies to prevent transmission of Candida auris in healthcare settings","volume":"17","author":"Ahmad","year":"2023","journal-title":"Curr. 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