{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:23:14Z","timestamp":1791256994792,"version":"4.1.0"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T00:00:00Z","timestamp":1704240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      A number of mosquito-borne viruses (MBVs), such as dengue virus (DENV), zika virus (ZIKV), chikungunya (CHIKV), West Nile virus (WNV), and yellow fever virus (YFV) exert adverse health impacts on the global population.\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      and\n                      <jats:italic>Aedes albopictus<\/jats:italic>\n                      are the prime vectors responsible for the transmission of these viruses. The viruses have acquired a number of routes for successful transmission, including horizontal and vertical transmission. Transovarial transmission is a subset\/type of vertical transmission adopted by mosquitoes for the transmission of viruses from females to their offspring through eggs\/ovaries. It provides a mechanism for these MBVs to persist and maintain their lineage during adverse climatic conditions of extremely hot and cold temperatures, during the dry season, or in the absence of susceptible vertebrate host when horizontal transmission is not possible.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The publications discussed in this systematic review were searched for using the PubMed, Scopus, and Web of Science databases, and websites such as those of the World Health Organization (WHO) and the European Centre for Disease Prevention and Control, using the search terms \u201ctransovarial transmission\u201d and \u201cmosquito-borne viruses\u201d from 16 May 2023 to 20 September 2023.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 2,391 articles were searched, of which 123 were chosen for full text evaluation, and 60 were then included in the study after screening and removing duplicates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The present systematic review focuses on understanding the above diseases, their pathogenesis, epidemiology and host\u2013parasite interactions. The factors affecting transovarial transmission, potential implications, mosquito antiviral defense mechanism, and the control strategies for these mosquito-borne viral diseases (MBVDs) are also be included in this review.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2023.1304938","type":"journal-article","created":{"date-parts":[[2024,1,2]],"date-time":"2024-01-02T23:30:12Z","timestamp":1704238212000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":27,"title":["Transovarial transmission of mosquito-borne viruses: a systematic review"],"prefix":"10.3389","volume":"13","author":[{"given":"Sangeeta","family":"Janjoter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divya","family":"Kataria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahima","family":"Yadav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisha","family":"Dahiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelam","family":"Sehrawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,1,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006822","article-title":"Alternative strategies for mosquito-borne arbovirus control","volume":"13","author":"Achee","year":"2019","journal-title":"PloS Negl. Trop. Dis."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002990","article-title":"Evidence of experimental vertical transmission of emerging novel ECSA genotype of Chikungunya Virus in Aedes aegypti","volume":"8","author":"Agarwal","year":"2014","journal-title":"PloS Negl. Trop. Dis."},{"key":"B3","doi-asserted-by":"publisher","first-page":"28792","DOI":"10.1038\/srep28792","article-title":"The w Mel strain of Wolbachia reduces transmission of Zika virus by Aedes aegypti","volume":"6","author":"Aliota","year":"2016","journal-title":"Sci. Rep."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004677","article-title":"The w Mel strain of Wolbachia reduces transmission of chikungunya virus in Aedes aegypti","volume":"10","author":"Aliota","year":"2016","journal-title":"PloS Negl. Trop. Dis."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02050","article-title":"Aedes aegypti molecular responses to Zika virus: modulation of infection by the toll and jak\/stat immune pathways and virus host factors","volume":"8","author":"Angler\u00f3-Rodr\u00edguez","year":"2017","journal-title":"Front. Microbiol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"173","DOI":"10.3390\/insects9040173","article-title":"The ecological significance and implications of transovarial transmission among the vector-borne Bunyaviruses: a review","volume":"9","author":"Bergren","year":"2018","journal-title":"Insects"},{"key":"B7","doi-asserted-by":"publisher","first-page":"20200119","DOI":"10.1098\/rspb.2020.0119","article-title":"Potential for Zika virus transmission by mosquitoes in temperate climates","volume":"287","author":"Blagrove","year":"2020","journal-title":"Proc. R. Soc. B"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058824","article-title":"Effects of fluctuating daily temperatures at critical thermal extremes on Aedes aegypti life-history traits","volume":"8","author":"Carrington","year":"2013","journal-title":"PloS one"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047350","article-title":"Comparative expression profiles of midgut genes in dengue virus refractory and susceptible Aedes aegypti across critical period for virus infection","volume":"7","author":"Chauhan","year":"2012","journal-title":"PLoS ONE"},{"key":"B10","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.pt.2015.09.009","article-title":"Mosquito defense strategies against viral infection","volume":"32","author":"Cheng","year":"2016","journal-title":"Trends Parasitol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1016\/j.pt.2005.02.007","article-title":"Melanization immune responses in mosquito vectors","volume":"21","author":"Christensen","year":"2005","journal-title":"Trends Parasitol."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3389\/fitd.2022.1059283","article-title":"ZIKV: Epidemiology, infection mechanism and current therapeutics","volume":"3","author":"Dahiya","year":"2023","journal-title":"Front. Trop. Dis."},{"key":"B13","doi-asserted-by":"publisher","first-page":"37","DOI":"10.4103\/0972-9062.331411","article-title":"Zika virus vertical transmission in mosquitoes: A less understood mechanism","volume":"59","author":"Dahiya","year":"2022","journal-title":"J. Vector Borne Dis."},{"key":"B14","volume-title":"Introduction to modern virology","author":"Dimmock","year":"2015"},{"key":"B15","doi-asserted-by":"publisher","first-page":"511","DOI":"10.3390\/insects3020511","article-title":"New insights into control of arbovirus replication and spread by insect RNA interference pathways","volume":"3","author":"Donald","year":"2012","journal-title":"Insects"},{"key":"B16","unstructured":"2023"},{"key":"B17","doi-asserted-by":"publisher","first-page":"698","DOI":"10.2174\/1389203711209070620","article-title":"Insect inducible antimicrobial peptides and their applications","volume":"14","author":"Ezzati-Tabrizi","year":"2013","journal-title":"Curr. Protein Pept. Sci."},{"key":"B18","doi-asserted-by":"publisher","first-page":"3741","DOI":"10.3390\/v7072795","article-title":"Tissue barriers to arbovirus infection in mosquitoes","volume":"7","author":"Franz","year":"2015","journal-title":"Viruses"},{"key":"B19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/jme\/tjv168","article-title":"How important is vertical transmission of dengue viruses by mosquitoes (Diptera: Culicidae)","volume":"53","author":"Grunnill","year":"2016","journal-title":"J. Med. entomology"},{"key":"B20","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1016\/S0188-4409(02)00378-8","article-title":"The global emergence\/resurgence of arboviral diseases as public health problems","volume":"33","author":"Gubler","year":"2002","journal-title":"Arch. Med. Res."},{"key":"B21","first-page":"67","article-title":"Patient-based dengue virus surveillance in Aedes aegypti from Recife, Brazil","volume":"47","author":"Guedes","year":"2010","journal-title":"J. Vector. Borne Dis."},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008362","article-title":"Evidence of transovarial transmission of Chikungunya and Dengue viruses in field-caught mosquitoes in Kenya","volume":"14","author":"Heath","year":"2020","journal-title":"PloS Negl. Trop. Dis."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1075362","article-title":"Effects of constant temperature and daily fluctuating temperature on the transovarial transmission and life cycle of Aedes albopictus infected with Zika virus","volume":"13","author":"Jian","year":"2023","journal-title":"Front. Microbiol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"158","DOI":"10.4269\/ajtmh.2002.67.158","article-title":"Persistence of dengue-3 virus through transovarial transmission passage in successive generations of Aedes aegypti mosquitoes","volume":"67","author":"Joshi","year":"2002","journal-title":"Am. J. Trop. Med. hygiene"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005187","article-title":"Engineered Aedes aegypti JAK\/STAT pathway-mediated immunity to dengue virus","volume":"11","author":"Jupatanakul","year":"2017","journal-title":"PloS Negl. Trop. Dis."},{"key":"B26","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1007\/s13199-022-00836-1","article-title":"Role of vertically transmitted viral and bacterial endosymbionts of Aedes mosquitoes. Does Paratransgenesis influence vector-borne disease control","volume":"86","author":"Kaavya","year":"2022","journal-title":"Symbiosis"},{"key":"B27","doi-asserted-by":"publisher","first-page":"1336","DOI":"10.1093\/jme\/tjac048","article-title":"Natural Aedes-borne virus infection detected in male adult Aedes aegypti (Diptera: Culicidae) collected from urban settings in M\u00e9rida, Yucat\u00e1n, M\u00e9xico","volume":"59","author":"Kirstein","year":"2022","journal-title":"J. Med. Entomology"},{"key":"B28","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.cell.2009.03.045","article-title":"The canonical Notch signaling pathway: unfolding the activation mechanism","volume":"137","author":"Kopan","year":"2009","journal-title":"Cell"},{"key":"B29","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1016\/j.coviro.2015.10.003","article-title":"Dissecting vectorial capacity for mosquito-borne viruses","volume":"15","author":"Kramer","year":"2015","journal-title":"Curr. Opin. Virol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"765","DOI":"10.3390\/idr14050078","article-title":"Study of dengue virus transovarial transmission in aedes spp. in ternate city using streptavidin-biotin-peroxidase complex immunohistochemistry","volume":"14","author":"Kurnia","year":"2022","journal-title":"Infect. Dis. Rep."},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008776","article-title":"Vertical transmission of zika virus in Aedes albopictus","volume":"14","author":"Lai","year":"2020","journal-title":"PloS Negl. Trop. Dis."},{"key":"B32","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1111\/j.1365-2567.2007.02576.x","article-title":"Distinct signalling pathways promote phagocytosis of bacteria, latex beads and lipopolysaccharide in medfly haemocytes","volume":"121","author":"Lamprou","year":"2007","journal-title":"Immunology"},{"key":"B33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-019-3433-8","article-title":"Mosquito antiviral defense mechanisms: A delicate balance between innate immunity and persistent viral infection","volume":"12","author":"Lee","year":"2019","journal-title":"Parasites Vectors"},{"key":"B34","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1016\/j.meegid.2014.07.025","article-title":"Vertical transmission of arboviruses in mosquitoes: a historical perspective","volume":"28","author":"Lequime","year":"2014","journal-title":"Infection Genet. Evol."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005548","article-title":"Determinants of arbovirus vertical transmission in mosquitoes","volume":"12","author":"Lequime","year":"2016","journal-title":"PloS Pathog."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/emi.2017.8","article-title":"Vector competence and transovarial transmission of two Aedes aegypti strains to Zika virus","volume":"6","author":"Li","year":"2017","journal-title":"Emerging Microbes infections"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0277276","article-title":"Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India","volume":"17","author":"Lole","year":"2022","journal-title":"PloS One"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001252","article-title":"Induction of a peptide with activity against a broad spectrum of pathogens in the Aedes aegypti salivary gland, following infection with dengue virus","volume":"7","author":"Luplertlop","year":"2011","journal-title":"PloS Pathog."},{"key":"B39","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1673\/031.007.5501","article-title":"Infection and vertical transmission of Kamiti river virus in laboratory bred Aedes aegypti mosquitoes","volume":"7","author":"Lutomiah","year":"2007","journal-title":"J. Insect Sci."},{"key":"B40","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1093\/jme\/tjab197","article-title":". Biology and transmission dynamics of Aedes flavivirus","volume":"59","author":"Peinado","year":"2022","journal-title":"J. Med. entomology"},{"key":"B41","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.chom.2018.02.001","article-title":"Dicer-2-dependent generation of viral DNA from defective genomes of RNA viruses modulates antiviral immunity in insects","volume":"23","author":"Poirier","year":"2018","journal-title":"Cell Host Microbe"},{"key":"B42","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.chom.2016.05.015","article-title":"Zika virus infects human placental macrophages","volume":"20","author":"Quicke","year":"2016","journal-title":"Cell Host Microbe"},{"key":"B43","doi-asserted-by":"publisher","first-page":"1108","DOI":"10.4269\/ajtmh.1983.32.1108","article-title":"Transovarial Transmission of Dengue Viruses by Mosquitoes: Aedes albopictus and Aedes aegypti","volume":"32","author":"Rosen","year":"1983","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"B44","doi-asserted-by":"publisher","first-page":"517","DOI":"10.15294\/kemas.v17i4.28007","article-title":"Detection of dengue virus transovarial transmission in dengue hemorrhagic fever endemic areas","volume":"17","author":"Saepudin","year":"2022","journal-title":"KEMAS: Jurnal Kesehatan Masyarakat"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006754","article-title":"Demonstration of efficient vertical and venereal transmission of dengue virus type-2 in a genetically diverse laboratory strain of Aedes aegypti","volume":"12","author":"S\u00e1nchez-Vargas","year":"2018","journal-title":"PloS Negl. Trop. Dis."},{"key":"B46","doi-asserted-by":"publisher","first-page":"1753","DOI":"10.1016\/j.cellsig.2014.02.024","article-title":"Nodulation: an unexplored cellular defense mechanism in insects","volume":"26","author":"Satyavathi","year":"2014","journal-title":"Cell. Signalling"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00801","article-title":"De novo DNA synthesis in Aedes aegypti midgut cells as a complementary strategy to limit dengue viral replication","volume":"9","author":"Serrato-Salas","year":"2018","journal-title":"Front. Microbiol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1111\/nyas.13306","article-title":"Zika and chikungunya: mosquito\u2010borne viruses in a changing world","volume":"1399","author":"Shragai","year":"2017","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"B49","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/0169-4758(91)90283-T","article-title":"Transovarial transmission","volume":"7","author":"Smith","year":"1991","journal-title":"Parasitol. Today"},{"key":"B50","doi-asserted-by":"publisher","first-page":"17841","DOI":"10.1073\/pnas.0905006106","article-title":"An evolutionary conserved function of the JAK-STAT pathway in anti-dengue defense","volume":"106","author":"Souza-Neto","year":"2009","journal-title":"Proc. Natl. Acad. Sci."},{"key":"B51","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1007\/s12038-008-0063-2","article-title":"Chikungunya: an overview","volume":"33","author":"Sudeep","year":"2008","journal-title":"J. Biosci."},{"key":"B52","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.4269\/ajtmh.16-0448","article-title":"Vertical transmission of Zika virus in Aedes aegypti mosquitoes","volume":"95","author":"Thangamani","year":"2016","journal-title":"Am. J. Trop. Med. hygiene"},{"key":"B53","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1111\/j.1948-7134.2011.00151.x","article-title":"Prospective field study of transovarial dengue-virus transmission by two different forms of Aedes aegypti in an urban area of Bangkok, Thailand","volume":"36","author":"Thongrungkiat","year":"2011","journal-title":"J. Vector Ecol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1089\/vbz.2016.1940","article-title":"Detection of Sindbis and Inkoo virus RNA in genetically typed mosquito larvae sampled in Northern Sweden","volume":"16","author":"Tingstr\u00f6m","year":"2016","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"B55","doi-asserted-by":"publisher","first-page":"112002","DOI":"10.1016\/j.chaos.2022.112002","article-title":"A periodic Chikungunya model with virus mutation and transovarial transmission","volume":"158","author":"Wang","year":"2022","journal-title":"Chaos Solitons Fractals"},{"key":"B56","unstructured":""},{"key":"B57","unstructured":"2022"},{"key":"B58","unstructured":"2022"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000098","article-title":"The Aedes aegypti toll pathway controls dengue virus infection","volume":"4","author":"Xi","year":"2008","journal-title":"PloS Pathog."},{"key":"B60","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1007\/s10142-023-01072-6","article-title":"Mosquito gene targeted RNAi studies for vector control","volume":"23","author":"Yadav","year":"2023","journal-title":"Funct. Integr. Genomics"},{"key":"B61","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.mbs.2017.01.006","article-title":"The transovarial transmission in the dynamics of dengue infection: Epidemiological implications and thresholds","volume":"286","author":"Yang","year":"2017","journal-title":"Math. Biosci."},{"key":"B62","doi-asserted-by":"publisher","first-page":"586","DOI":"10.3390\/insects11090586","article-title":"A Toll-Sp\u00e4tzle pathway in the immune response of Bombyx mori","volume":"11","author":"Yu","year":"2020","journal-title":"Insects"},{"key":"B63","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1111\/j.1348-0421.1993.tb03232.x","article-title":"Transovarial transmission of chikungunya virus by Aedes albopictus mosquitoes ingesting microfilariae of Dirofilaria immitis under laboratory conditions","volume":"37","author":"Zytoon","year":"1993","journal-title":"Microbiol. Immunol."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2023.1304938\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,2]],"date-time":"2024-01-02T23:30:17Z","timestamp":1704238217000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2023.1304938\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,3]]},"references-count":63,"alternative-id":["10.3389\/fcimb.2023.1304938"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2023.1304938","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,3]]},"article-number":"1304938"}}