{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T16:00:43Z","timestamp":1792425643745,"version":"4.5.0"},"reference-count":62,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T00:00:00Z","timestamp":1711584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Investigations assessing the value of metagenomic next-generation sequencing (mNGS) for distinguish\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      infection from colonization are currently insufficient.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The performance of mNGS in distinguishing\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      infection from colonization, along with the differences in patients\u2019 characteristics, antibiotic adjustment, and lung microbiota, were analyzed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The abundance of\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      significantly differed between patients with\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      infection (n=36) and colonization (n=32) (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.0001). Receiver operating characteristic (ROC) curve result for bronchoalveolar lavage fluid (BALF) mNGS indicated an area under the curve of 0.894 (95%CI: 0.811-0.976), with an optimal threshold value of 23 for discriminating between\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      infection and colonization. The infection group exhibited a higher proportion of antibiotic adjustments in comparison to the colonization group (50% vs. 12.5%,\n                      <jats:italic>P<\/jats:italic>\n                      = 0.001), with antibiotic escalation being more dominant. Age, length of hospital stay, hemoglobin, cough and chest distress were significantly positively correlated with\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      infection. The abundance of\n                      <jats:italic>A. fumigatus<\/jats:italic>\n                      and Epstein-Barr virus (EBV) significantly increased in the infection group, whereas the colonization group exhibited higher abundance of\n                      <jats:italic>A. niger<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      BALF mNGS is a valuable tool for differentiating between colonization and infection of\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      . Variations in patients\u2019 age, length of hospital stay, hemoglobin, cough and chest distress are observable between patients with\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      infection and colonization.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2024.1345706","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T00:32:01Z","timestamp":1711585921000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":29,"title":["Clinical performance of metagenomic next-generation sequencing for diagnosis of pulmonary Aspergillus infection and colonization"],"prefix":"10.3389","volume":"14","author":[{"given":"Ziwei","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Gai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafeng","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuru","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geriletu","family":"Ao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahuan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danni","family":"Shu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianbing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhidan","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,3,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjresp-2022-001358","article-title":"Clinical characteristics, diagnosis, outcomes and lung microbiome analysis of invasive pulmonary aspergillosis in the community-acquired pneumonia patients","volume":"10","author":"Ao","year":"2023","journal-title":"BMJ Open Respir. 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