{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T12:46:15Z","timestamp":1792759575171,"version":"4.5.0"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T00:00:00Z","timestamp":1709683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K07031, 22K15675"],"award-info":[{"award-number":["21K07031, 22K15675"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Wickerhamiella<\/jats:italic> is a genus of budding yeast that is mainly isolated from environmental samples, and 40 species have been detected. The yeast isolated from human clinical samples usually only contain three species: <jats:italic>W. infanticola<\/jats:italic>, <jats:italic>W. pararugosa<\/jats:italic> and <jats:italic>W. sorbophila<\/jats:italic>. In this study, we isolated <jats:italic>W. tropicalis<\/jats:italic> from a blood sample of a six-year-old female with a history of B-cell precursor lymphoblastic leukemia in Japan in 2022. Though the strain was morphologically identified as <jats:italic>Candida<\/jats:italic> species by routine microbiological examinations, it was subsequently identified as <jats:italic>W. tropicalis<\/jats:italic> by sequencing the internal transcribed spacer (ITS) of ribosomal DNA (rDNA). The isolate had amino acid substitutions in ERG11 and FKS1 associated with azole and echinocandin resistance, respectively, in <jats:italic>Candida<\/jats:italic> species and showed intermediate-resistant to fluconazole and micafungin. The patient was successfully treated with micafungin. Furthermore, matrix-assisted laser desorption\/ionization mass spectrometry (MALDI-MS) detected three novel peaks that are specific for <jats:italic>W. tropicalis<\/jats:italic>, indicating that MALDI-MS analysis is useful for rapid detection of <jats:italic>Wickerhamiella<\/jats:italic> species in routine microbiological examinations.<\/jats:p>","DOI":"10.3389\/fcimb.2024.1361432","type":"journal-article","created":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T15:30:51Z","timestamp":1709739051000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Isolation and identification of Wickerhamiella tropicalis from blood culture by MALDI-MS"],"prefix":"10.3389","volume":"14","author":[{"given":"Satomi","family":"Takei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanae","family":"Teramoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Fujimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megumi","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Fukui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Sekiguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaaki","family":"Kawakami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayoshi","family":"Chonan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Wakita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Horiuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Miida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Naito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruo","family":"Kirikae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Tada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Tabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,3,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1182","DOI":"10.1111\/j.1365-2672.2007.03631.x","article-title":"Ascomycetous yeast species recovered from grapes damaged by honeydew and sour rot","volume":"104","author":"Barata","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"5315","DOI":"10.1093\/bioinformatics\/btac672","article-title":"GTDB-Tk v2: memory friendly classification with the genome taxonomy database","volume":"38","author":"Chaumeil","year":"2022","journal-title":"Bioinformatics"},{"key":"B3","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard-4th ed CLSI Document M27-A4","year":"2017"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fox054","article-title":"Flowers as a reservoir of yeast diversity: description of Wickerhamiella nectarea f.a. sp. nov., and Wickerhamiella natalensis f.a. sp. nov. from South African flowers and pollinators, and transfer of related Candida species to the genus Wickerhamiella as new combinations","volume":"17","author":"de Vega","year":"2017","journal-title":"FEMS Yeast. Res."},{"key":"B5","first-page":"289","article-title":"Performance of a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory","volume":"55","author":"Eigner","year":"2009","journal-title":"Clin. Lab."},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.1283","article-title":"Candida pararugosa: first reported bloodstream infection in an adult","volume":"9","author":"El Helou","year":"2017","journal-title":"Cureus"},{"key":"B7","doi-asserted-by":"publisher","first-page":"2715","DOI":"10.1099\/00221287-145-10-2715","article-title":"Multiple amino acid substitutions in lanosterol 14alpha-demethylase contribute to azole resistance in Candida albicans","volume":"145","author":"Favre","year":"1999","journal-title":"Microbiol. (Reading)."},{"key":"B8","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1016\/j.resmic.2004.04.003","article-title":"Candida pararugosa isolation from the oral cavity of an Italian denture wearer","volume":"155","author":"Giammanco","year":"2004","journal-title":"Res. Microbiol."},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11090963","article-title":"Overview on the infections related to rare candida species","volume":"11","author":"Kumar","year":"2022","journal-title":"Pathogens"},{"key":"B10","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/s10482-009-9399-3","article-title":"The use of parsimony network analysis for the formal delineation of phylogenetic species of yeasts: Candida apicola, Candida azyma, and Candida parazyma sp. nov., cosmopolitan yeasts associated with floricolous insects","volume":"97","author":"Lachance","year":"2010","journal-title":"Antonie. Van. Leeuwenhoek."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1431","DOI":"10.1099\/00207713-48-4-1431","article-title":"Wickerhamiella australiensis, Wickerhamiella cacticola, Wickerhamiella occidentalis, Candida drosophilae and Candida lipophila, five new related yeast species from flowers and associated insects","author":"Lachance","year":"1998","journal-title":"Int. J. Syst. Bacteriol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"e180566","DOI":"10.1590\/0074-02760180566","article-title":"Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches","volume":"114","author":"Maciel","year":"2019","journal-title":"Mem. Inst. Oswaldo. Cruz."},{"key":"B13","doi-asserted-by":"publisher","first-page":"e0097820","DOI":"10.1128\/mSphere.00978-20","article-title":"Molecular epidemiology of drug-resistant mycobacterium tuberculosis in Japan","volume":"6","author":"Mizukoshi","year":"2021","journal-title":"mSphere"},{"key":"B14","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1080\/13693780310001597674","article-title":"Recurrent isolation of an uncommon yeast, Candida pararugosa, from a sarcoma patient","volume":"42","author":"Nakagawa","year":"2004","journal-title":"Med. Mycol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"239","DOI":"10.2323\/jgam.53.239","article-title":"Candida kazuoi sp. nov. and Candida hasegawae sp. nov., two new species of ascomycetous anamorphic yeasts isolated from insect frass in Thailand","volume":"53","author":"Nakase","year":"2007","journal-title":"J. Gen. Appl. Microbiol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1007\/s42770-023-00985-5","article-title":"Catheter-associated blood stream infections due to Candida pararugosa in a patient with acute myeloid leukemia: A case report","volume":"54","author":"Nasri","year":"2023","journal-title":"Braz. J. Microbiol."},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.3390\/jof6040194","article-title":"Rare invasive yeast infections in greek neonates and children, a retrospective 12-year study","volume":"6","author":"Noni","year":"2020","journal-title":"J. Fungi. (Basel)."},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00547-18","article-title":"Echinocandin-induced microevolution of candida parapsilosis influences virulence and abiotic stress tolerance","volume":"3","author":"Papp","year":"2018","journal-title":"mSphere"},{"key":"B19","doi-asserted-by":"publisher","first-page":"S69","DOI":"10.4103\/0974-8490.150528","article-title":"Performance of chromogenic media for Candida in rapid presumptive identification of Candida species from clinical materials","volume":"7","author":"Pravin Charles","year":"2015","journal-title":"Pharmacognosy. Res."},{"key":"B20","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1186\/s13059-020-02155-4","article-title":"Estimating the quality of eukaryotic genomes recovered from metagenomic analysis with EukCC","volume":"21","author":"Saary","year":"2020","journal-title":"Genome Biol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"2596","DOI":"10.1099\/ijsem.0.004075","article-title":"Wickerhamiella osmotolerans sp. nov. and Wickerhamiella tropicalis sp. nov., novel ascomycetous yeast in the family Wickerhamiellaceae","volume":"70","author":"Sakpuntoon","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0210397","article-title":"Analysis of antifungal resistance genes in Candida albicans and Candida glabrata using next generation sequencing","volume":"14","author":"Spettel","year":"2019","journal-title":"PloS One"},{"key":"B23","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1093\/mmycol\/myu016","article-title":"Uncommon opportunistic yeast bloodstream infections from Qatar","volume":"52","author":"Taj-Aldeen","year":"2014","journal-title":"Med. Mycol."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.3390\/jof7020079","article-title":"Candida albicans-the virulence factors and clinical manifestations of infection","volume":"7","author":"Talapko","year":"2021","journal-title":"J. Fungi. (Basel)."},{"key":"B25","doi-asserted-by":"publisher","first-page":"612","DOI":"10.2183\/pjab.95.042","article-title":"Classification of Cutibacterium acnes at phylotype level by MALDI-MS proteotyping","volume":"95","author":"Teramoto","year":"2019","journal-title":"Proc. Jpn. Acad. Ser. B. Phys. Biol. Sci."},{"key":"B26","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/bf02578847","article-title":"The yeast genus Wickerhamiella gen. nov. (Ascomycetes)","volume":"39","author":"van der Walt","year":"1973","journal-title":"Antonie. Van. Leeuwenhoek."},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3390\/jof6040276","article-title":"Candidemia in children with Malignancies: report from the infection working group of the hellenic society of pediatric hematology-oncology","volume":"6","author":"Vasileiou","year":"2020","journal-title":"J. Fungi. (Basel)."},{"key":"B28","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.simyco.2016.11.007","article-title":"DNA barcoding analysis of more than 9\u2008000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation","volume":"85","author":"Vu","year":"2016","journal-title":"Stud. Mycol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1007\/s10482-008-9238-y","article-title":"Candida alocasiicola sp. nov., Candida hainanensis sp. nov., Candida heveicola sp. nov. and Candida musiphila sp. nov., novel anamorphic, ascomycetous yeast species isolated from plants","volume":"94","author":"Wang","year":"2008","journal-title":"Antonie. Van. Leeuwenhoek."},{"key":"B30","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1186\/s12917-021-02821-0","article-title":"Isolation and identification of Candida tropicalis in sows with fatal infection: a case report","volume":"17","author":"Zhai","year":"2021","journal-title":"BMC Vet. Res."},{"key":"B31","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1128\/jcm.02543-13","article-title":"Yeast identification algorithm based on use of the Vitek MS system selectively supplemented with ribosomal DNA sequencing: proposal of a reference assay for invasive fungal surveillance programs in China","volume":"52","author":"Zhang","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.3390\/jof6040330","article-title":"Implementation of MALDI-TOF mass spectrometry and peak analysis: application to the discrimination of cryptococcus neoformans species complex and their interspecies hybrids","volume":"6","author":"Zvezdanova","year":"2020","journal-title":"J. Fungi. (Basel)."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2024.1361432\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T15:30:56Z","timestamp":1709739056000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2024.1361432\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,6]]},"references-count":32,"alternative-id":["10.3389\/fcimb.2024.1361432"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2024.1361432","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,6]]},"article-number":"1361432"}}