{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T08:32:04Z","timestamp":1761294724535,"version":"3.41.2"},"reference-count":68,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T00:00:00Z","timestamp":1713225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Diagnosing <jats:italic>Mycoplasma faucium<\/jats:italic> poses challenges, and it's unclear if its rare isolation is due to infrequent occurrence or its fastidious nutritional requirements.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study analyzes the complete genome sequence of <jats:italic>M. faucium<\/jats:italic>, obtained directly from the pus of a sternum infection in a lung transplant patient using metagenomic sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Genome analysis revealed limited therapeutic options for the <jats:italic>M. faucium<\/jats:italic> infection, primarily susceptibility to tetracyclines. Three classes of mobile genetic elements were identified: two new insertion sequences, a new prophage (phiUMCG-1), and a species-specific variant of a mycoplasma integrative and conjugative element (MICE). Additionally, a Type I Restriction-Modification system was identified, featuring 5\u2019-terminally truncated <jats:italic>hsdS<\/jats:italic> pseudogenes with overlapping repeats, indicating the potential for forming alternative <jats:italic>hsdS<\/jats:italic> variants through recombination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study represents the first-ever acquisition of a complete circularized bacterial genome directly from a patient sample obtained from invasive infection of a primary sterile site using culture-independent, PCR-free clinical metagenomics.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2024.1368923","type":"journal-article","created":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T05:01:07Z","timestamp":1713243667000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["The complete genome sequence of unculturable Mycoplasma faucium obtained through clinical metagenomic next-generation sequencing"],"prefix":"10.3389","volume":"14","author":[{"given":"Artur J.","family":"Sabat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Durfee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Schuyler","family":"Baldwin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktoria","family":"Akkerboom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Voss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander W.","family":"Friedrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik","family":"Bathoorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,4,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1093\/cid\/ciaa089","article-title":"First report of candidatus mycoplasma haemohominis infection in Australia causing persistent fever in an animal carer","volume":"72","author":"Alcorn","year":"2021","journal-title":"Clin. 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