{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:48:46Z","timestamp":1788371326839,"version":"build-2803163510"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The emergence of ESBLs producing cephalosporin-resistant <jats:italic>Escherichia coli<\/jats:italic> isolates poses a threat to public health. This study aims to decipher the genetic landscape and gain insights into ESBL-producing <jats:italic>E. coli<\/jats:italic> strains belonging to the high-risk clone ST410 from pediatric patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>29 <jats:italic>E. coli<\/jats:italic> ST410 isolates were collected from young children and subjected to antimicrobial susceptibility testing, Whole-genome sequencing (WGS), serotype analysis, MLST, ESBL genes, virulence genes, and plasmid profiling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Antimicrobial susceptibility testing demonstrated a high level of resistance to cephalosporins followed by aminoglycoside, sulfonamide, carbapenem and penicillin group of antibiotics. However, n=20\/29 shows MDR phenotype. Phylogenetic group B2 (n=15) dominated, followed by group D (n=7), group A (n=4), and group B1 (n=3). Serotyping analysis identified O1:H7 (n=8), O2:H1 (n=6), O8:H4 (n=5), O16:H5 (n=4), and O25:H4 (n=3). Other serotypes identified included O6:H1, O15:H5, and O18:H7 (n=1 each). The most commonly detected ESBL genes were <jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub>, (n=26), followed by <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub> (n=23), and <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub> (n=18). Additionally, <jats:italic>bla<\/jats:italic><jats:sub>OXA-1<\/jats:sub> (n=10), <jats:italic>bla<\/jats:italic><jats:sub>OXA-48<\/jats:sub> (n=5), <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub> (n=3), <jats:italic>bla<\/jats:italic><jats:sub>KPC-3<\/jats:sub> (n=2), <jats:italic>bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub> (n=4), <jats:italic>bla<\/jats:italic><jats:sub>NDM-5<\/jats:sub> (n=1), <jats:italic>bla<\/jats:italic><jats:sub>GES-1<\/jats:sub> (n=2), <jats:italic>bla<\/jats:italic><jats:sub>GES-5<\/jats:sub> (n=1), and <jats:italic>bla<\/jats:italic><jats:sub>CYM-1<\/jats:sub> (n=3). Notable virulence genes identified within the ST410 isolates included <jats:italic>fimH<\/jats:italic> (n=29), <jats:italic>papC<\/jats:italic> (n=24), <jats:italic>hlyA<\/jats:italic> (n=22), and <jats:italic>cnf1<\/jats:italic> (n=18), among others. Diverse plasmids were observed including IncFIS, IncX4, IncFIA, IncCol, IncI2 and IncFIC with transmission frequency ranges from 1.3X10<jats:sup>-2<\/jats:sup> to 2.7X10<jats:sup>-3<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The ST410 clone exhibited a complex resistance profile, diverse serotypes, the presence of specific resistance genes (ESBL genes), virulence gene repertoire, and diverse plasmids. The <jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub> was the most prevalent ESBL gene detected.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2024.1403234","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T05:10:25Z","timestamp":1726031425000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Genetic landscape of ESBL producing international clone ST410 of Escherichia coli from pediatric infections in Shenzhen, China"],"prefix":"10.3389","volume":"14","author":[{"given":"Sandip","family":"Patil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Pai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunsheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xinye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaowei","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanket","family":"Kaushik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Silvester","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sixi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiqiu","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,9,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis","volume":"399","year":"2022","journal-title":"Lancet"},{"key":"B2","doi-asserted-by":"publisher","first-page":"e00047","DOI":"10.1128\/CMR.00047-19","article-title":"Epidemiology of \u03b2-lactamase-producing pathogens","volume":"33","author":"Bush","year":"2020","journal-title":"Clin. 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