{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:47:14Z","timestamp":1789620434389,"version":"build-2803163510"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T00:00:00Z","timestamp":1721952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Babesia ovis<\/jats:italic>\n                    , transmitted by\n                    <jats:italic>Rhipicephalus bursa<\/jats:italic>\n                    ticks, is the causative agent of ovine babesiosis, a disease characterized by fever, anemia, hemoglobinuria, and high mortality in sheep. This study investigates whether sheep that survived babesiosis without treatment can serve as a source of infection for\n                    <jats:italic>B. ovis<\/jats:italic>\n                    -free host-seeking\n                    <jats:italic>R. bursa<\/jats:italic>\n                    larvae in a later season. Three donor sheep were experimentally infected with\n                    <jats:italic>B. ovis<\/jats:italic>\n                    , and after six months, persistence of\n                    <jats:italic>B. ovis<\/jats:italic>\n                    was assessed through blood and tick transmission experiments. Blood from donor sheep was intravenously injected into three recipient sheep, while donor sheep were also infested with\n                    <jats:italic>B. ovis<\/jats:italic>\n                    -free\n                    <jats:italic>R. bursa<\/jats:italic>\n                    larvae. Engorged nymphs molted to adults, and new recipient sheep were infested with these ticks. All recipient sheep were monitored for\n                    <jats:italic>B. ovis<\/jats:italic>\n                    for 100 days using microscopic, serological, and molecular approaches. The presence of\n                    <jats:italic>B. ovis<\/jats:italic>\n                    was confirmed in the recipient sheep that received blood, leading to clinical infection in two. However, no\n                    <jats:italic>B. ovis<\/jats:italic>\n                    was detected in recipient sheep infested with ticks. These results suggest that sheep recovering from\n                    <jats:italic>B. ovis<\/jats:italic>\n                    infection do not serve as a source of infection for\n                    <jats:italic>R. bursa<\/jats:italic>\n                    larvae in subsequent seasons.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2024.1428719","type":"journal-article","created":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T01:10:29Z","timestamp":1721956229000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Role of Rhipicephalus bursa larvae in transstadial transmission and endemicity of Babesia ovis in chronically infected sheep"],"prefix":"10.3389","volume":"14","author":[{"given":"Recep","family":"Firat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Can","family":"Ulucesme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munir","family":"Aktas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onur","family":"Ceylan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferda","family":"Sevinc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reginaldo G.","family":"Bastos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos E.","family":"Suarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sezayi","family":"Ozubek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,7,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.vetpar.2005.05.057","article-title":"Development of a polymerase chain reaction method for diagnosis of Babesia ovis infection in sheep and goats","volume":"133","author":"Aktas","year":"2005","journal-title":"Vet. 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