{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:32:06Z","timestamp":1790890326292,"version":"4.1.0"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T00:00:00Z","timestamp":1731283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Candida albicans<\/jats:italic> (<jats:italic>C. albicans<\/jats:italic>, CA) is an essential invasive fungus in clinical diagnosis. Although several detection methods exist, none meet the need for early diagnosis. A rapid, sensitive, and specific diagnostic tool is crucial for effective prevention and control of <jats:italic>C. albicans<\/jats:italic> infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study aimed to develop a new, rapid, and ultrasensitive diagnostic tool for <jats:italic>C. albicans<\/jats:italic> detection based on restriction endonuclease-mediated real-time loop-mediated isothermal amplification (ERT-LAMP-CA). The ERT-LAMP-CA technology combines LAMP amplification, restriction endonuclease cleavage, and real-time fluorescence detection in a single reaction tube, which can complete a diagnosis of <jats:italic>C. albicans<\/jats:italic> in a short time (approximately 1 h).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Herein, we developed the primer sequences required for ERT-LAMP-CA based on the ITS2 gene of <jats:italic>C. albicans<\/jats:italic> and found that ERT-LAMP-CA limit of detection was approximately 500 ag\/\u03bcL genomic DNA and can present negative results for non-<jats:italic>C. albicans<\/jats:italic> templates. We tested sputum samples from 64 patients with suspected <jats:italic>C. albicans<\/jats:italic> infections to validate ERT-LAMP-CA applicability in clinical sample testing and found that ERT-LAMP-CA was consistent with multiplex PCR-capillary electrophoresis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>In conclusion, ERT-LAMP-CA is a rapid, accurate, and sensitive assay with excellent potential for clinical and basic laboratory diagnosis and an efficient screening strategy.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2024.1450199","type":"journal-article","created":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T06:10:20Z","timestamp":1731305420000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["A novel, rapid, ultrasensitive diagnosis platform for detecting Candida albicans using restriction endonuclease\u2010mediated real-time loop-mediated isothermal amplification"],"prefix":"10.3389","volume":"14","author":[{"given":"Yizhe","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingrun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,11,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1816","DOI":"10.3201\/eid2410.180649","article-title":"Candida auris in healthcare facilities, New York, USA 2013-2017","volume":"24","author":"Adams","year":"2018","journal-title":"Emerg. 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