{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:25:00Z","timestamp":1793118300068,"version":"4.5.0"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T00:00:00Z","timestamp":1734652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Mycoplasma hyopneumoniae<\/jats:italic>\n                    (\n                    <jats:italic>M. hyopneumoniae<\/jats:italic>\n                    ) is one of the primary agents involved in porcine respiratory disease complex, and circulates in the swine industry worldwide. The prevention and control of\n                    <jats:italic>M. hyopneumoniae<\/jats:italic>\n                    is complicated. Thus, a recombinase-aided amplification (RAA) assay coupled with the clustered regularly-interspaced short palindromic repeats (CRISPR)\/Cas12a system was established for the detection of\n                    <jats:italic>M. hyopneumoniae<\/jats:italic>\n                    . The most suitable primer pairs and CRISPR RNA (crRNA) were screened and selected for the RAA-CRISPR\/Cas12a detection system. We have achieved a detection limit of 1 copy\/\u00b5L and 5 copies\/\u00b5L per reaction for the RAA-CRISPR\/Cas12a-fluorescence assay and RAA-CRISPR\/Cas12a-lateral flow assay (LFA), respectively. Furthermore, the RAA-CRISPR\/Cas12a system displayed no cross-reactivity with other respiratory pathogens. The performance of the RAA-CRISPR\/Cas12a system was compared with PCR as recommended by the Chinese national standard (GB\/T 35909-2018) and qPCR as recommended by the Chinese entry\u2013exit inspection and quarantine industry standard (SN\/T4104-2015) for clinical samples, and good consistency with these methods was observed. Above all, the methods shed a light on the convenient, portable, visual, highly sensitive and specific detection of\n                    <jats:italic>M. hyopneumoniae<\/jats:italic>\n                    , demonstrating a great application potential for on-site monitoring of\n                    <jats:italic>M. hyopneumoniae<\/jats:italic>\n                    in the field.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2024.1469558","type":"journal-article","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T01:30:28Z","timestamp":1734658228000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Rapid detection of Mycoplasma hyopneumoniae by recombinase-aided amplification combined with the CRISPR\/Cas12a system"],"prefix":"10.3389","volume":"14","author":[{"given":"Kaili","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingyu","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changwen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyou","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caixia","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.1048861","article-title":"Porcine respiratory disease complex: dynamics of polymicrobial infections and management strategies after the introduction of the african swine fever","volume":"9","author":"Assavacheep","year":"2022","journal-title":"Front. 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