{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T06:00:12Z","timestamp":1778220012869,"version":"3.51.4"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The use of beneficial bacteria like <jats:italic>Lactobacillus<\/jats:italic> spp. is a potential innovative approach to fight antibiotic-resistant pathogens. <jats:italic>Klebsiella pneumoniae<\/jats:italic> is one of the most concerning multi drug-resistant (MDR) pathogens, and its ability to colonize the human gut is considered to be the main reason for recurrent infections in critically ill patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, <jats:italic>Lactobacillus gasseri<\/jats:italic> 1A-TV, already described for its probiotic activity, was characterized at the genomic level. Moreover, its cell-free supernatant (CFS) was tested for antimicrobial activity against extended-spectrum \u03b2-lactamase (ESBL)- and carbapenemase (KPC)-producing <jats:italic>K. pneumoniae<\/jats:italic> clinical isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Whole-genome sequencing showed that the <jats:italic>L. gasseri<\/jats:italic> 1A-TV genome was of 2,018,898 bp in size with 34.9% GC content, containing 1,937 putative protein coding sequences, 55 tRNA, and 4 rRNA detected by RAST and classified in 20 functional groups by Cluster of Orthologous Genes (COG). BAGEL4 (BActeriocin GEnome minimal tooL) and the antiSMASH 7.0 pipeline identified two bacteriocin biosynthetic gene clusters (BBGCs), namely, BBGC1 that comprises two class IIc bacteriocins including gassericin A-like bacteriocin, and BBGC2 carrying the class III bacteriocin helveticin J. Strikingly, 1A-TV CFS inhibited the growth of all <jats:italic>K. pneumoniae<\/jats:italic> isolates only after 8 h of incubation, showing a bactericidal effect at 24 h and interfering, even at lower concentrations, with the biofilm production of biofilm-producer strains independently of a bactericidal effect. NMR analysis of CFS identified and quantified several metabolites involved in carbohydrate metabolism and amino acid metabolism, and organic acids like ethanol, lactate, acetate, and succinate. Finally, <jats:italic>in vitro<\/jats:italic> assays of 1A-TV showed significant co-aggregation effects against carbapenem-resistant <jats:italic>K. pneumoniae<\/jats:italic>, namely, strains 1, 2, 3, and 7.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings highlight the antimicrobial activity of 1A-TV as a probiotic candidate or its CFS as a natural bioproduct active against MDR <jats:italic>K. pneumoniae<\/jats:italic> strains, underlining the importance of novel therapeutic strategies for prevention and control of ESBL- and carbapenemase-producing <jats:italic>K. pneumoniae<\/jats:italic> colonization.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2024.1471107","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T06:30:33Z","timestamp":1733207433000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Cell-free supernatant of Lactobacillus gasseri 1A-TV shows a promising activity to eradicate carbapenem-resistant Klebsiella pneumoniae colonization"],"prefix":"10.3389","volume":"14","author":[{"given":"Gaia","family":"Vertillo Aluisio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Lina","family":"Mezzatesta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviana","family":"Cafiso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Scuderi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania","family":"Stefani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Santagati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,12,3]]},"reference":[{"key":"B1","unstructured":"Clinical microbiology procedures handbook\n          \n          2024"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fsufs.2022.887642","article-title":"Postbiotics: From emerging concept to application","volume":"6","author":"Aggarwal","year":"2022","journal-title":"Front. 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