{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T15:17:12Z","timestamp":1789139832594,"version":"build-2803163510"},"reference-count":90,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T00:00:00Z","timestamp":1728518400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P 36130"],"award-info":[{"award-number":["P 36130"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Although the importance of the microbiome in the context of tick biology and vector competence has recently come into a broader research focus, the field is still in its infancy and the complex ecological interactions between the tick residential bacteria and pathogens are obscure. Here, we show that an environmentally acquired gut bacterium has the potential to impair <jats:italic>Borrelia afzelii<\/jats:italic> colonization within the tick vector through a secreted metalloprotease. Oral introduction of either <jats:italic>Bacillus cereus<\/jats:italic> LTG-1 isolate or its purified enhancin (<jats:italic>Bc<\/jats:italic>Enhancin) protein significantly reduces <jats:italic>B. afzelii<\/jats:italic> burden in the guts of <jats:italic>Ixodes ricinus<\/jats:italic> ticks. This effect is attributed to the ability of <jats:italic>Bc<\/jats:italic>Enhancin to degrade a glycan-rich peritrophic matrix (PM), which is a gut protective barrier essential for <jats:italic>Borrelia<\/jats:italic> survival. Our study highlights the importance of the gut microbiome in determining tick vector competence and provides a deeper mechanistic insight into the complex network of interactions between <jats:italic>Borrelia<\/jats:italic>, the tick, and the tick microbiome.<\/jats:p>","DOI":"10.3389\/fcimb.2024.1476266","type":"journal-article","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T11:45:08Z","timestamp":1728560708000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["A metalloprotease secreted by an environmentally acquired gut bacterium hinders Borrelia afzelii colonization in Ixodes ricinus"],"prefix":"10.3389","volume":"14","author":[{"given":"Adnan","family":"Hod\u017ei\u0107","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gorana","family":"Veinovi\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amer","family":"Ali\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Seki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kunert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgi","family":"Nikolov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratko","family":"Sukara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jovana","family":"\u0160upi\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sne\u017eana","family":"Tomanovi\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Berry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,10,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"E781","DOI":"10.1073\/pnas.1613422114","article-title":"Pathogen-mediated manipulation of arthropod microbiota to promote infection","volume":"114","author":"Abraham","year":"2017","journal-title":"Proc. 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