{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:58:33Z","timestamp":1792126713471,"version":"4.4.0"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T00:00:00Z","timestamp":1731369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Klebsiella michiganensis<\/jats:italic> is an emerging hospital-acquired bacterial pathogen, particularly strains harboring plasmid-mediated carbapenemase genes. Here, we recovered and characterized a multidrug-resistant strain, <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub>\u2013producing <jats:italic>Klebsiella michiganensis<\/jats:italic> LS81, which was isolated from the abdominal drainage fluid of a clinical patient in China, and further characterized the co-harboring plasmid. <jats:italic>K. michiganensis<\/jats:italic> LS81 tested positive for the <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub> genes by PCR sequencing, with <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub> located on a plasmid as confirmed by S1 nuclease pulsed-field gel electrophoresis combined with Southern blotting. In the transconjugants, the <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub> genes were successfully transferred to the recipient strain <jats:italic>E. coli<\/jats:italic> EC600. Whole-genome sequencing and bioinformatics analysis confirmed that this strain belongs to sequence type 196 (ST196), with a complete genome comprising a 5,926,662bp circular chromosome and an 81,451bp IncM2 plasmid encoding <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub> (designated pLS81-KPC). The IncM2 plasmid carried multiple \u03b2-lactamase genes such as <jats:italic>bla<\/jats:italic><jats:sub>TEM-1B<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-3<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub> inserted in truncated Tn<jats:italic>6296<\/jats:italic> with the distinctive core structure IS<jats:italic>Kpn27<\/jats:italic>\u2013<jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub>\u2013IS<jats:italic>Kpn6<\/jats:italic>. A comparison with 46 <jats:italic>K. michiganensis<\/jats:italic> genomes available in the NCBI database revealed that the closest phylogenetic relative of <jats:italic>K. michiganensis<\/jats:italic> LS81 is a clinical isolate from a wound swab in the United Kingdom. Ultimately, the pan-genomic analysis unveiled a substantial accessory genome within the strain, alongside significant genomic plasticity within the <jats:italic>K. michiganensis<\/jats:italic> species, emphasizing the necessity for continuous surveillance of this pathogen in clinical environments.<\/jats:p>","DOI":"10.3389\/fcimb.2024.1492700","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T06:15:45Z","timestamp":1731392145000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Genomic characterization of a blaKPC-2\u2013producing IncM2 plasmid harboring transposon \u0394Tn6296 in Klebsiella michiganensis"],"prefix":"10.3389","volume":"14","author":[{"given":"Jian-Mei","family":"Song","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu-Bo","family":"Long","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao-Rui","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-Juan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Chun","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Ling","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Wei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Gang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Yao","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Li","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,11,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1016\/j.ijantimicag.2018.08.011","article-title":"Insights into a novel Tn4401 deletion (Tn4401i) in a multidrug-resistant Klebsiella pneumoniae clinical strain belonging to the high-risk clonal group 258 producing KPC-2","volume":"52","author":"Ara\u00fajo","year":"2018","journal-title":"Int. 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