{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T11:55:44Z","timestamp":1783943744052,"version":"3.55.0"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T00:00:00Z","timestamp":1733270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"Background<\/jats:title>Zhuanggu Shubi ointment (ZGSBG) has good efficacy in postmenopausal osteoporosis (PMO), but the mechanism of efficacy involving gut microecology has not been elucidated.<\/jats:p><\/jats:sec>Objective<\/jats:title>This study investigated the mechanism of ZGSBG in regulating gut microecology in PMO.<\/jats:p><\/jats:sec>Methods<\/jats:title>The bilateral ovarian denervation method was used to construct a rat model of PMO and was administered ZGSBG. Behavior, bone transformation, gut microbiota, intestinal mucosal barrier, and intestinal inflammatory-related indexes were detected.<\/jats:p><\/jats:sec>Results<\/jats:title>After ZGSBG intervention, bone R-hydroxy glutamic acid protein and procollagen type I N-terminal propeptides were significantly upregulated, while C-terminal telopeptide of type-I collagen and tartrate-resistant acid phosphatase-5b were significantly downregulated. Pathological analysis demonstrated an improvement in femoral and colonic structures. The expressions of zonula occludens-1, occludin, claudin-1, and secretory immunoglobulin A in the colonic tissues were significantly elevated, while the levels of tumor necrosis factor-\u03b1, interleukin-1\u03b2, interleukin-6, and lipopolysaccharides were reduced. Moreover, characteristic bacteria Muribaculaceae<\/jats:italic> and Prevotella<\/jats:italic> were significantly enriched. Furthermore, Muribaculaceae<\/jats:italic> and Prevotella<\/jats:italic> have a positive correlation with intestinal mucosal barrier function and a negative correlation with intestinal inflammatory responses.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>ZGSBG promoted bone formation, inhibited bone resorption, regulated gut microbiota, repaired intestinal mucosal barrier damage, and inhibited intestinal inflammatory responses in PMO rats. Muribaculaceae<\/jats:italic> and Prevotella<\/jats:italic> might play positive roles in ZGSBG treatment of intestinal mucosal barrier injury and inflammatory reactions in PMO.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2024.1500111","type":"journal-article","created":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T06:46:17Z","timestamp":1733294777000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Zhuanggu Shubi ointment mediated the characteristic bacteria-intestinal mucosal barrier-bone metabolism axis to intervene in postmenopausal osteoporosis"],"prefix":"10.3389","volume":"14","author":[{"given":"Xiaoya","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Pei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueying","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,12,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1996830","DOI":"10.1080\/21688370.2021.1996830","article-title":"Epithelial integrity, junctional complexes, and biomarkers associated with intestinal functions","volume":"10","author":"Alizadeh","year":"2022","journal-title":"Tissue. 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