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Microbiol."],"abstract":"<jats:p>Attachment and uptake into host cells are pivotal steps in the life cycle of the <jats:italic>Chlamydiaceae<\/jats:italic>, a family of obligate intracellular pathogens. <jats:italic>Chlamydia trachomatis (Ctr)<\/jats:italic> possesses a family of nine polymorphic membrane proteins (Pmps), which have been shown to be crucial for adhesion and internalization. However, the host-cell molecules involved have so far remained unknown. Here, we show that a fragment of <jats:italic>Ctr<\/jats:italic> PmpD, which forms high-molecular-weight oligomers in solution and adheres to epithelial cells, also binds to secreted clusterin (sCLU), a chaperone-like protein that is secreted into the extracellular space by the host cell, and forms part of the chaperone- and receptor-mediated extracellular protein degradation (CRED) pathway. Using <jats:italic>in vitro<\/jats:italic> assays, we demonstrate that sCLU interacts directly with soluble rPmpD. In infection experiments, depletion of sCLU from the culture medium leads to a significant decrease in <jats:italic>Ctr<\/jats:italic> infection. Thus, sCLU is the first host-cell interaction partner identified for a <jats:italic>Ctr<\/jats:italic> Pmp and the first case in which sCLU has been shown to be a vital component for the establishment of a bacterial infection.<\/jats:p>","DOI":"10.3389\/fcimb.2024.1519883","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T06:42:52Z","timestamp":1737960172000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["The secreted host-cell protein clusterin interacts with PmpD and promotes Chlamydia trachomatis infection"],"prefix":"10.3389","volume":"14","author":[{"given":"Fabienne","family":"Kocher","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes H.","family":"Hegemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,1,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1016\/j.femsre.2005.03.002","article-title":"The chlamydial developmental cycle","volume":"29","author":"Abdelrahman","year":"2005","journal-title":"FEMS Microbiol. 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