{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:32:09Z","timestamp":1790890329974,"version":"4.1.0"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:00:00Z","timestamp":1738540800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"\n Introduction<\/jats:title>\n \n Candida albicans<\/jats:italic>\n , a human fungal pathogen, multiplies to invade body cells and causes fungal diseases in the condition of insufficient body's immune function. Early detection of\n C. albicans<\/jats:italic>\n is required to guide appropriate prevention and treatment.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The purpose of this study was to establish a\n C. albicans<\/jats:italic>\n assay based on newly developed closed dumbbell-mediated isothermal amplification (CDA) to achieve rapid and simple point of care diagnostic. The CDA technique was carried out by specific primers targeting at the conserved\n C. albicans<\/jats:italic>\n ITS2 gene. All primers were selected and evaluated by real-time fluorescence monitoring and endpoint visual judgement indicated by hydroxy naphthol blue (HNB). Optimal primers and accelerate primers (out primers and loop primers) were designed and selected after confirmation of the fundamental CDA primers to achieve more efficient CDA reaction for\n C. albicans<\/jats:italic>\n detection (CA-OL-CDA).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n After establishment of the assay, 9\n non-Candida albicans<\/jats:italic>\n strains, including 3\n Candida<\/jats:italic>\n species were tested to negative by adopting the established CA-OL-CDA assay, indicated high specificity. The limit of detection of\n Candida albicans<\/jats:italic>\n DNA by CA-OL-CDA assay was 6.2\u00d710\n -6<\/jats:sup>\n ng\/\u03bcL of DNA (10 copies\/\u03bcL), 10-fold more sensitive than real-time quantitative PCR (qPCR).\n <\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n \n The CA-OL-CDA assay exhibited advantages of high specificity, sensitivity, simpler and more efficient operation. In addition, the CA-OL-CDA method holds potential in on-site detection for\n C. albicans<\/jats:italic>\n using color shift by adopting the reaction mixture based on HNB.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fcimb.2025.1484089","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T01:33:22Z","timestamp":1738546402000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Rapid and simple detection of Candida albicans using closed dumbbell-mediated isothermal amplification"],"prefix":"10.3389","volume":"15","author":[{"given":"Yanli","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuhan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeling","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guifang","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,2,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007717","article-title":"Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection","volume":"15","author":"Bertolini","year":"2019","journal-title":"PLoS Pathog."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1818","DOI":"10.1016\/j.burns.2021.02.025","article-title":"The impact of simultaneous inoculation of Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans on rodent burn wounds","volume":"47","author":"Brandenburg","year":"2021","journal-title":"Burns"},{"key":"B3","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.micpath.2018.02.028","article-title":"Candida albicans - Biology, molecular characterization, pathogenicity, and advances in diagnosis and control - An update","volume":"117","author":"Dadar","year":"2018","journal-title":"Microb. 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