{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:49:49Z","timestamp":1753890589545,"version":"3.41.2"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T00:00:00Z","timestamp":1743379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Malaria, caused by <jats:italic>Plasmodium vivax<\/jats:italic>, remains a major global health problem, particularly in tropical and subtropical regions. This study aimed to investigate the potential of <jats:italic>Pv<\/jats:italic>Vir14-derived peptides to improve diagnostic accuracy for <jats:italic>P. vivax<\/jats:italic> infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The reactivity of specific <jats:italic>Pv<\/jats:italic>Vir14-derived peptides against sera from acutely infected individuals from endemic and non-endemic areas experiencing a <jats:italic>P. vivax<\/jats:italic> malaria outbreak was assessed, as well as their sensitivity, specificity, and immunodominance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the eight tested peptides (H08, K09, I03, G08, F08, H04, L06, and N04), H08, G08, and L06 showed significantly higher IgG reactivity in sera from individuals living in endemic areas, mainly within those with multiple malaria episodes. After a <jats:italic>P. vivax<\/jats:italic> outbreak in a non-endemic area, H08 and G08 had the highest IgM frequencies, however, sensitivity and specificity analyses indicated that L06 was the most effective peptide for identifying infected individuals. Depletion ELISA confirmed the immunodominance of L06, G08, and H08 and showed a significant reduction in IgG reactivity to <jats:italic>Pv<\/jats:italic>Vir14. The peptides L06, G08, and H08, showed high sensitivity and specificity as diagnostic tools for <jats:italic>P. vivax<\/jats:italic> malaria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These peptides can improve diagnostic accuracy, especially in endemic areas, providing better support to malaria control and management programs.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2025.1484863","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T06:49:58Z","timestamp":1743403798000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Immunogenicity of PvVir14-derived peptides to improve the serological diagnosis of Plasmodium vivax infection"],"prefix":"10.3389","volume":"15","author":[{"given":"Ramayana Morais de Medeiros","family":"Brito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raianna F.","family":"Fantin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Laura","family":"Grossi de Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Rafaela Antunes","family":"Porto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabela de Brito","family":"Duval","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Bryan da Rocha","family":"Rihs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian Maria Lapa Montenegro","family":"Pimentel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata Maria Costa","family":"Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elainne Christine de Souza","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseli de Oliveira","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniella Castanheira","family":"Batholomeu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Toshio","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian Lacerda","family":"Bueno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"35","DOI":"10.4269\/ajtmh.16-0171","article-title":"Diagnosis and treatment of Plasmodium vivax malaria","volume":"95","author":"Baird","year":"2016","journal-title":"Am. 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