{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T16:51:04Z","timestamp":1772211064559,"version":"3.50.1"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010814","name":"Anhui Provincial Department of Education","doi-asserted-by":"publisher","award":["2023AH053286"],"award-info":[{"award-number":["2023AH053286"]}],"id":[{"id":"10.13039\/501100010814","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the diagnostic and prognostic value of miR-9-5p in peripheral blood mononuclear cells in sepsis patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Differentially expressed miR-9-5p in sepsis were screened from a database and available literature. Subsequently, iBMDM cell validation was conducted and the expression level of miR-9-5p in peripheral blood mononuclear cells was determined using RT-qPCR in 69 sepsis patients and 30 non-sepsis patients with infections, 24 hours after ICU admission. A control group that comprised 35 healthy individuals, matched for age and sex, was set up from those who agreed to provide blood samples laboratory analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>On admission to the ICU, the levels of miR-9-5p were significantly higher in sepsis patients (10.13 [3.21, 24.94]) than in non-sepsis patients with infections (2.08 [1.68, 3.18]) and healthy controls (0.86 [0.36, 1.88]) (p &amp;lt; 0.001). The miR-9-5p levels were positively correlated with the severity of the disease as indicated by the SOFA score (r=0.656, P&amp;lt;0.001). The AUC of miR-9-5p in predicting sepsis, septic shock, and in-hospital death was 0.825, 0.821, and 0.845, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Elevated expression levels of miR-9-5p in peripheral blood mononuclear cells are significantly associated with an increased risk of sepsis and septic shock, and also indicate a higher risk of organ dysfunction.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2025.1509496","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T05:25:57Z","timestamp":1745299557000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Analysis of the diagnostic and prognostic value of Peripheral blood mononuclear cell microRNA-9-5p in patients with sepsis in the intensive care unit"],"prefix":"10.3389","volume":"15","author":[{"given":"Haoming","family":"Ye","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruojue","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Ji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianru","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinquan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Limian","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Shao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,4,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0262339","article-title":"Serum miR-34a-5p and miR-199a-3p as new biomarkers of neonatal sepsis","volume":"17","author":"Abdelaleem","year":"2022","journal-title":"PLoS One"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25126476","article-title":"MicroRNA as sepsis biomarkers: A comprehensive review","volume":"25","author":"Bindayna","year":"2024","journal-title":"Int. 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