{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T03:04:47Z","timestamp":1793675087420,"version":"4.5.1"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>The spread of extended-spectrum beta-lactamase (ESBL)- and carbapenemase-producing <jats:italic>Escherichia coli<\/jats:italic> clones in humans, animals, and the environment is of great concern worldwide. In this study, we characterized four carbapenemase-producing <jats:italic>E. coli<\/jats:italic> (CP-Ec) isolated from human samples. Two isolates of ST401, rarely associated with carbapenemase and\/or ESBL resistance genes, harbored <jats:italic>bla<\/jats:italic>KPC-3 and <jats:italic>bla<\/jats:italic>VIM-1 genes, respectively, and were genetically distant from each other. One CP-Ec isolate belonging to ST355, typically found in poultry and environmental sources and not associated with carbapenemases, was <jats:italic>bla<\/jats:italic>KPC-3 positive and showed a wide range of virulence genes. The last CP-Ec strain belonged to ST3564, previously described in livestock with a large virulome but no carbapenemase. The CP-Ec ST3564 isolate co-harbored <jats:italic>bla<\/jats:italic>VIM-1 and <jats:italic>bla<\/jats:italic>NDM-7 genes, which, to our knowledge, have not been previously reported in Italy. These results emphasize the crucial role of a genomic-based surveillance program to intercept the emergence of critical <jats:italic>E. coli<\/jats:italic> clones.<\/jats:p>","DOI":"10.3389\/fcimb.2025.1519827","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T07:08:38Z","timestamp":1741936118000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Polyclonal carbapenemase-producing Escherichia coli in Northern Italy: the emergence of NDM-7"],"prefix":"10.3389","volume":"15","author":[{"given":"Vittoria","family":"Mattioni Marchetti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Corbella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurora","family":"Piazza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Gaiarsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Mileto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Merla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Kuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aseel","family":"AbuAlshaar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Cambieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Migliavacca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fausto","family":"Baldanti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1111\/1758-2229.13076","article-title":"Molecular and metabolic characteristics of wastewater associated Escherichia coli strains","volume":"14","author":"Behruznia","year":"2022","journal-title":"Environ. 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