{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T10:47:59Z","timestamp":1770288479597,"version":"3.49.0"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>To investigate the prevalence of <jats:italic>Tritrichomonas foetus<\/jats:italic>, <jats:italic>Pentatrichomonas hominis<\/jats:italic>, <jats:italic>Giardia intestinalis<\/jats:italic>, <jats:italic>Cryptosporidium<\/jats:italic>, <jats:italic>Microsporidium<\/jats:italic>, and <jats:italic>Sarcocystis<\/jats:italic> in domestic cats in Anhui Province, China, and their potential role as zoonotic hosts for human infection, a total of 304 fecal samples from two different sources were screened for the presence of related pathogens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using microscopy, along with polymerase chain reaction (PCR) or nested PCR amplification, followed by genotyping through sequence analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The infection rates of <jats:italic>T. foetus<\/jats:italic>, <jats:italic>P. hominis<\/jats:italic>, <jats:italic>G. intestinalis<\/jats:italic>, <jats:italic>Cryptosporidium<\/jats:italic>, <jats:italic>Enterocytozoon bieneusi<\/jats:italic>, and <jats:italic>Sarcocystis<\/jats:italic> were 5.6%, 0%, 1.7%, 0.7%, 2.6%, and 0%, respectively. The evolutionary relationships and genetic characteristics of <jats:italic>G. intestinalis<\/jats:italic> based on the GDH gene, <jats:italic>Cryptosporidium<\/jats:italic> based on the SSU rRNA gene, and <jats:italic>E. bieneusi<\/jats:italic> based on the ITS sequence were assessed: five cases of <jats:italic>G. intestinalis<\/jats:italic> were identified, with four belonging to assemblage F and one to zoonotic assemblage B, two <jats:italic>Cryptosporidium<\/jats:italic> cases were identified as <jats:italic>Cryptosporidium felis<\/jats:italic>, and all eight <jats:italic>E. bieneusi<\/jats:italic> cases were identified as belonging to group 1, with three cases being genotype D, three EbpA, and two EbpC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Age, neutering status, and deworming were identified as potential risk factors. Further analysis revealed that diarrhea, as a clinical symptom, could serve as an indicator for pathogen infection. Although the pathogen infection rates detected in this study were relatively low, their zoonotic transmission potential cannot be ignored. Therefore, special attention should be paid, and it is essential to establish targeted prevention plans.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2025.1522176","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T06:45:59Z","timestamp":1737960359000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Gastrointestinal protozoa in pet cats from Anhui province: prevalence and molecular characterization"],"prefix":"10.3389","volume":"15","author":[{"given":"Hao","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Tong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhonghui","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congshan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyang","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,1,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1186\/s12917-022-03520-0","article-title":"Gastrointestinal parasites of cats in Egypt: high prevalence high zoonotic risk","volume":"18","author":"Abbas","year":"2022","journal-title":"BMC Veterinary Res."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1007\/s00436-011-2564-4","article-title":"Molecular evidence for person-to-person transmission of a novel subtype in Giardia duodenalis assemblage B at the rehabilitation institution for developmentally disabled people","volume":"110","author":"Abe","year":"2012","journal-title":"Parasitol. 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