{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T02:43:16Z","timestamp":1790995396263,"version":"4.1.0"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Previous studies have suggested that dietary organic iron offers health advantages compared to its inorganic counterpart. However, the effects of iron hydroxy methionine analog chelate (Fe-HMA) supplementation in weaned piglets have not been fully explored. Therefore, this study aimed to investigate the effects of replacing ferrous sulfate with Fe-HMA as the iron source on serum biochemistry, antioxidant capacity, and gut microbiota in weaned piglets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>One hundred and twenty weaned piglets were randomly allocated to two treatment groups. Each group contained four replicates, with 15 pigs per replicate. Piglets were fed either 100 mg Fe\/kg in the form of ferrous sulfate (Fe-sulfate group) or 50 mg Fe\/kg in the form of Fe-HMA (Fe-HMA group) as the iron source for 28 days.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and discussion<\/jats:title>\n                    <jats:p>Results showed that supplementing Fe-HMA as an iron source significantly increased the levels of triglycerides and glucose in portal venous serum, albumin in both serum and portal venous serum and decreased serum low-density lipoprotein level in weaned piglets. Additionally, Fe-HMA supplementation significantly reduced serum and liver malondialdehyde levels, while increasing catalase (CAT), glutathione peroxidase (GSH-Px), total superoxide dismutase, and manganese superoxide dismutase levels in serum, as well as GSH-Px and CAT levels in the liver. Moreover, Fe-HMA regulated the intestinal microbiota composition, notably increasing the relative abundance of Proteobacteria and decreasing microbes involved in aromatic_compound_degradation. In conclusion, dietary replacing inorganic iron with Fe-HMA improved metabolic parameters and antioxidant capacity, and regulated gut microbiota composition in weaned piglets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2025.1539607","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T01:54:44Z","timestamp":1739843684000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Changes of metabolic parameters, antioxidant capacity, and gut microbiota in response to substitution of ferrous sulfate with iron hydroxy methionine analog chelate in weaned piglets"],"prefix":"10.3389","volume":"15","author":[{"given":"Yuemeng","family":"Fu","sequence":"first","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, College of Animal Science and Technology, Shandong Agricultural University","place":["Tai\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohui","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, College of Animal Science and Technology, Shandong Agricultural University","place":["Tai\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhang","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, College of Animal Science and Technology, Shandong Agricultural University","place":["Tai\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejun","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shandong Agricultural University","place":["Tai\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Jiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, College of Animal Science and Technology, Shandong Agricultural University","place":["Tai\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbiao","family":"Lu","sequence":"additional","affiliation":[{"name":"Research and Development Department, Fujian Syno Biotech Co., Ltd.","place":["Fuzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiren","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, College of Animal Science and Technology, Shandong Agricultural University","place":["Tai\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,2,18]]},"reference":[{"key":"B1","unstructured":"Biagi\n              G.\n            \n            \n              Cinti\n              E.\n            \n            \n              Ferruzza\n              S.\n            \n            \n              Mordenti\n              A.\n            \n            \n              Predieri\n              G.\n            \n            \n              Tegoni\n              M.\n            \n          \n          DEU\n          Metal chelates of 2-hydroxy-4-methylthiobutanoic acid in animal feeding: characterization, \n          \n          2004"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1093\/jas\/skx062","article-title":"Weaning disrupts intestinal antioxidant status, impairs intestinal barrier and mitochondrial function, and triggers mitophagy in piglets1","volume":"96","author":"Cao","year":"2018","journal-title":"J. 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