{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:48:11Z","timestamp":1753890491841,"version":"3.41.2"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T00:00:00Z","timestamp":1746144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170947, 32400094"],"award-info":[{"award-number":["82170947, 32400094"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","award":["2025ZNSFSC0771"],"award-info":[{"award-number":["2025ZNSFSC0771"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"Objective<\/jats:title>To elucidate the microbial diversities and compositions of dental plaque in children with severe early childhood caries (S-ECC) before and after comprehensive dental treatment under general anesthesia, contributing evidence for the reestablishment of oral microecological balance.<\/jats:p><\/jats:sec>Design<\/jats:title>Twenty children aged 2.2-5.5 years diagnosed with S-ECC without systemic diseases were enrolled in this study. Dental plaque samples were collected before and after treatment, analyzed using high-throughput sequencing on the Illumina Miseq platform. Bioinformatics methods were employed to compare the dental plaque microbial diversity and compositions differences before and after comprehensive dental treatment under general anesthesia.<\/jats:p><\/jats:sec>Results<\/jats:title>Significant enhancements in dental plaque microbial diversity were observed post-comprehensive treatment (P<\/jats:italic> &lt; 0.05). At the phylum level, the relative abundance of Actinobacteria significantly decreased after treatment (P<\/jats:italic> &lt; 0.05). At the genus level, the relative abundance of Streptococcus<\/jats:italic>, Neisseria<\/jats:italic>, and Rothia<\/jats:italic> significantly declined (P<\/jats:italic> &lt; 0.05), while Prevotella<\/jats:italic> showed a significant increase after treatment (P<\/jats:italic> &lt; 0.05).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Following comprehensive dental treatment under general anesthesia, children with S-ECC exhibit significant changes in the microbial diversity and composition of dental plaque, indicating a shift towards a more balanced oral microecological state. This study highlights the importance of dental intervention in positively altering oral microbiota.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2025.1541785","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T11:48:58Z","timestamp":1746186538000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbial shifts in dental plaque of children with severe early childhood caries following comprehensive dental treatment under general anesthesia"],"prefix":"10.3389","volume":"15","author":[{"given":"Xinyue","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qizhao","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,5,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1128\/JCM.40.3.1001-1009.2002","article-title":"Molecular analysis of bacterial species associated with childhood caries","volume":"40","author":"Becker","year":"2002","journal-title":"J. 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