{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T00:55:08Z","timestamp":1793235308346,"version":"4.5.0"},"reference-count":81,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012478","name":"Secretaria de Ciencia y Tecnolog\u00eda - Universidad Nacional de C\u00f3rdoba","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The increasing resistance to antimicrobial drugs has prompted global efforts to combat pathogenic bacteria and fungi. The World Health Organization\u2019s recent report underscores the urgent need for innovative antimicrobial strategies to address infections caused by\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      ,\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      ,\n                      <jats:italic>Candida albicans<\/jats:italic>\n                      , and\n                      <jats:italic>Candida tropicalis<\/jats:italic>\n                      . This study presents a comparative evaluation of the effects of biogenically synthesized zinc nanoparticles (ZnNPs) from\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      , highlighting their effectiveness against both planktonic and sessile forms of these tested pathogens.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The antimicrobial effects were assessed using the Kirby-Bauer disk diffusion method, broth microdilution, and time-kill assays. Biofilm formation and eradication were evaluated through crystal violet staining, resazurin assays, and colony-forming unit quantification. Additionally, the oxidative and nitrosative stress toxicity mechanisms triggered by ZnNPs, particularly those related to cellular stress, were investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results demonstrated that ZnNPs exhibit concentration-dependent inhibitory effects on both prokaryotic and eukaryotic microorganisms. ZnNPs inhibit biofilm formation by up to 50% in\n                      <jats:italic>E. coli<\/jats:italic>\n                      and yeast species, and up to 80% in\n                      <jats:italic>S. aureus<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These antibiofilm activities were attributed to disruptions in cellular stress metabolism, primarily driven by nitrosative stress through enhanced production of reactive nitrogen intermediates. ZnNPs synthesized through green methods offer significant advantages due to their biocompatibility and potential biomedical applications. These findings advance our understanding of ZnNPs in combating biofilm-associated infections, offering promising strategies to address pathogenic bacteria and fungi, which pose a critical threat to global health.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2025.1545119","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T05:25:19Z","timestamp":1752470719000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":29,"title":["Antibacterial, antifungal, and antibiofilm activities of biogenic zinc nanoparticles against pathogenic microorganisms"],"prefix":"10.3389","volume":"15","author":[{"given":"Eliana Daniela","family":"Lopez Venditti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina\u00a0Fernanda","family":"Crespo Andrada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela Soledad","family":"Bustos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Maldonado Torales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iv\u00e1n","family":"Manrrique Hughes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Gabriela","family":"Paraje","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Gui\u00f1az\u00fa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/ma14226983","article-title":"Green synthesis of zinc oxide nanoparticles (ZnO-NPs) by Pseudomonas aeruginosa and their activity against pathogenic microbes and common house mosquito, Culex pipiens","volume":"14","author":"Abdo","year":"2021","journal-title":"Materials (Basel)."},{"key":"B2","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.cbi.2018.03.008","article-title":"Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route","volume":"286","author":"Agarwal","year":"2018","journal-title":"Chem. 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