{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T15:36:03Z","timestamp":1769528163828,"version":"3.49.0"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Coxsackievirus A6 (CVA6) ranks as a primary enterovirus associated with hand-foot-mouth disease (HFMD) and herpangina (HA). Given its significant role in these diseases, there is an urgent need for an efficient identification method. This study presents a novel visual approach based on the reverse transcription polymerase spiral reaction (RT-PSR) for the rapid detection of CVA6. We designed an RT-PSR assay that targets and amplifies a segment of the VP1 gene. Hydroxy naphthol blue (HNB) is incorporated as the detection agent in this assay. To evaluate the performance of the RT-PSR assay, we analyzed 142 clinical throat swab samples. The results were benchmarked against those obtained using quantitative reverse transcription - polymerase chain reaction (qRT - PCR). The RT-PSR assay operates at 65\u00b0C for 60 minutes and exhibits a detection limit of 10 copies\/\u03bcL. When tested against other viruses, it consistently yielded negative results, demonstrating its high specificity. Moreover, the RT - PSR assay showed excellent agreement with a commercial qRT - PCR kit. In conclusion, by using HNB as an indicator, the RT - PSR assay emerges as a straightforward and highly sensitive method for detecting CVA6 in symptomatic throat samples. This approach holds great potential for improving the diagnosis and surveillance of CVA6 - related diseases.<\/jats:p>","DOI":"10.3389\/fcimb.2025.1563495","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T05:29:08Z","timestamp":1747114148000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Visual detection of Coxsackievirus A6 using a reverse transcription polymerase spiral reaction method"],"prefix":"10.3389","volume":"15","author":[{"given":"Kun","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanhang","family":"Ai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Longying","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiwei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guojun","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He","family":"Zha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Lei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shifei","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaifeng","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,5,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.656699","article-title":"Molecular epidemiology and clinical features of enteroviruses-associated hand, foot, and mouth disease and herpangina outbreak in zunyi, China 2019","volume":"8","author":"Ai","year":"2021","journal-title":"Front. 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