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Cell. Infect. Microbiol."],"abstract":"<jats:p>Babesiosis, a zoonotic parasitic disease caused by <jats:italic>Babesia<\/jats:italic> protozoa, poses significant infection risks across mammalian species. Clinical manifestations in vertebrate hosts range from spontaneous abortion to fatal outcomes, with immunocompromised individuals potentially transmitting the pathogen through blood products or transplanted organs, thereby amplifying epidemiological risks. Effective disease management carries substantial public health implications for livestock production, companion animal welfare, and food safety in endemic regions. In global endemic zones, conventional diagnostic approaches combine morphological identification of <jats:italic>Babesia<\/jats:italic> spp. with complementary serological assays. Contemporary molecular diagnostics, particularly nucleic acid amplification techniques, have emerged as valuable adjunctive tools. A critical challenge in veterinary practice involves persistent subclinical carriers among treated livestock populations, necessitating precise parasite speciation for effective transmission control. This review synthesizes recent advancements in babesiosis detection methodologies, with particular emphasis on their implementation in clinical microbiology laboratories. This article introduces the latest progress in Babesiosis detection technology and its application in clinical microbiology laboratories, to provide a theoretical and practical basis for the comprehensive prevention and control of Babesiosis.<\/jats:p>","DOI":"10.3389\/fcimb.2025.1575227","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T05:20:59Z","timestamp":1747372859000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Research progress on diagnostic techniques for different Babesia species in persistent infections"],"prefix":"10.3389","volume":"15","author":[{"given":"Zelin","family":"Jia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhilin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,5,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"033108","DOI":"10.1063\/1.4954196","article-title":"Insulator-based dielectrophoretic diagnostic tool for babesiosis","volume":"10","author":"Adekanmbi","year":"2016","journal-title":"Biomicrofluidics"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens8030143","article-title":"Diagnostic tools for the identification of babesia sp. in persistently infected cattle","volume":"8","author":"Alvarez","year":"2019","journal-title":"Pathogens"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2021.109479","article-title":"Recombinase polymerase amplification lateral flow dipstick (RPA-LF) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus 1758)","volume":"296","author":"An","year":"2021","journal-title":"Vet Parasitol."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2024.102351","article-title":"Hemopathogens in naturally infected bovine fetuses in Brazil","volume":"15","author":"Andrade","year":"2024","journal-title":"Ticks Tick Borne Dis."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2025.1498721","article-title":"Case report: First autochthonous Babesia vulpes infection in a dog from Italy","volume":"12","author":"Antognoni","year":"2025","journal-title":"Front. 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