{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:36:17Z","timestamp":1788568577522,"version":"build-2803163510"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T00:00:00Z","timestamp":1750377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>This study aimed to assess a novel lateral flow assay (LFA) for <jats:italic>Aspergillus<\/jats:italic> IgG detection in patients with non-neutropenic invasive aspergillosis (IA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>Aspergillus<\/jats:italic> IgG LFA and enzyme-linked immunosorbent assay (ELISA) were performed in non-neutropenic IA patients and control group (proven community acquired pneumonia and healthy persons), respectively. The diagnostic performance of <jats:italic>Aspergillus<\/jats:italic> IgG LFA for IA was evaluated and compared with ELISA method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>33 cases of acute IA, 30 cases of subacute IA and 80 controls were enrolled in this study. The level of plasma <jats:italic>Aspergillus<\/jats:italic> IgG LFA in the IA group was significantly higher than that in the control group (190.5 AU\/mL vs 50.3 AU\/mL, P &amp;lt;\u20090.001). In total, the sensitivity\/specificity\/PPV\/NPV of <jats:italic>Aspergillus<\/jats:italic> IgG LFA was 65.1%\/97.5%\/95.4%\/78.0%. The sensitivity and specificity of <jats:italic>Aspergillus<\/jats:italic> IgG LFA were equivalent to those of <jats:italic>Aspergillus<\/jats:italic> IgG ELISA with a 120 AU\/mL cut-off, but exhibited significantly higher specificity (97.5% vs 87.5%, P = 0.021) compared to the ELISA with an 80 AU\/mL cut-off. The consistency was strong among the two methods (P &amp;lt; 0.001, Kappa = 0.67\/0.68). The sensitivities\/specificities\/PPVs\/NPVs of <jats:italic>Aspergillus<\/jats:italic> IgG LFA were 57.6%\/97.5%\/90.5%\/84.8% for patients with acute IA, and 73.3%\/97.5%\/91.7%\/90.7% for patients with subacute IA, respectively. The \u201cany-positive\u201d strategy, which combined <jats:italic>Aspergillus<\/jats:italic> IgG LFA with sputum culture and serum galactomannan (GM), had a sensitivity\/specificity\/PPV\/NPV of 81.1%\/94.7%\/95.6%\/78.3%. The sensitivity\/specificity\/PPV\/NPV of bronchoalveolar lavage fluid (BALF) GM was 65.0%\/90.0%\/92.9%\/56.3%. When combined <jats:italic>Aspergillus<\/jats:italic> IgG LFA with BALF GM, the figures were 87.5%\/85.0%\/92.1%\/77.3%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Compared to the <jats:italic>Aspergillus<\/jats:italic> IgG ELISA, the <jats:italic>Aspergillus<\/jats:italic> IgG LFA exhibits comparable or superior diagnostic efficiency in IA patients, while offering a faster and more convenient option for clinical diagnosis. The \u201cany-positive\u201d strategy of combined diagnosis with <jats:italic>Aspergillus<\/jats:italic> IgG LFA serves as a valuable supplement to current diagnostic approaches, particularly benefiting patients who cannot tolerate invasive bronchoscopic procedures.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2025.1599425","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T01:22:32Z","timestamp":1750382552000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of a novel Aspergillus IgG lateral flow assay for the diagnosis of non-neutropenic patients with acute and subacute invasive aspergillosis"],"prefix":"10.3389","volume":"15","author":[{"given":"Yajie","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanhuan","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomin","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiamei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjin","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,6,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1007\/s11046-023-00749-7","article-title":"Diagnostic efficacy of Aspergillus galactomannan lateral flow assay in patients with hematological Malignancies: A prospective multicenter study","volume":"188","author":"Alhan","year":"2023","journal-title":"Mycopathologia"},{"key":"B2","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1093\/bmb\/ldad011","article-title":"The SSS revolution in fungal diagnostics: speed, simplicity and sensitivity","volume":"147","author":"Baker","year":"2023","journal-title":"Br. Med. Bull."},{"key":"B3","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1007\/s00134-024-07341-7","article-title":"Invasive Fungal Diseases in Adult Patients in Intensive Care Unit (FUNDICU): 2024 consensus definitions from ESGCIP, EFISG, ESICM, ECMM, MSGERC, ISAC, and ISHAM","volume":"50","author":"Bassetti","year":"2024","journal-title":"Intensive Care Med."},{"key":"B4","doi-asserted-by":"publisher","first-page":"E197","DOI":"10.1111\/1469-0691.12133","article-title":"Performance of two Aspergillus IgG EIA assays compared with the precipitin test in chronic and allergic aspergillosis","volume":"19","author":"Baxter","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"B5","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1128\/CMR.00055-08","article-title":"Pathogenesis of Aspergillus fumigatus in invasive aspergillosis","volume":"22","author":"Dagenais","year":"2009","journal-title":"Clin. Microbiol. Rev."},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000025233","article-title":"Comparing the diagnostic value of bronchoalveolar lavage fluid galactomannan, serum galactomannanan, and serum 1,3-beta-d-glucan in non-neutropenic respiratory disease patients with invasive pulmonary aspergillosis","volume":"100","author":"Dai","year":"2021","journal-title":"Med. (Baltimore)"},{"key":"B7","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1007\/s11046-023-00742-0","article-title":"Humoral immunity against Aspergillus fumigatus","volume":"188","author":"Delliere","year":"2023","journal-title":"Mycopathologia"},{"key":"B8","doi-asserted-by":"publisher","first-page":"e428","DOI":"10.1016\/S1473-3099(23)00692-8","article-title":"Global incidence and mortality of severe fungal disease","volume":"24","author":"Denning","year":"2024","journal-title":"Lancet Infect. Dis."},{"key":"B9","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1093\/cid\/ciz1008","article-title":"Revision and update of the consensus definitions of invasive fungal disease from the European organization for research and treatment of cancer and the mycoses study group education and research consortium","volume":"71","author":"Donnelly","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"B10","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1186\/s12890-023-02343-5","article-title":"Bronchoalveolar lavage fluid polymerase chain reaction for invasive pulmonary aspergillosis among high-risk patients: a diagnostic meta-analysis","volume":"23","author":"Han","year":"2023","journal-title":"BMC Pulm Med."},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00598-21","article-title":"Performance of the sona aspergillus galactomannan lateral flow assay in a cancer patient population","volume":"59","author":"Jani","year":"2021","journal-title":"J. Clin. Microbiol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1111\/myc.2019.62.issue-3","article-title":"Point-of-care diagnosis of invasive aspergillosis in non-neutropenic patients: Aspergillus Galactomannan Lateral Flow Assay versus Aspergillus-specific Lateral Flow Device test in bronchoalveolar lavage","volume":"62","author":"Jenks","year":"2019","journal-title":"Mycoses"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.631461","article-title":"The clinical characteristics of patients with nonneutropenic invasive pulmonary aspergillosis","volume":"8","author":"Liu","year":"2021","journal-title":"Front. Med. (Lausanne)"},{"key":"B14","doi-asserted-by":"publisher","first-page":"797.e791","DOI":"10.1016\/j.cmi.2023.02.002","article-title":"The clinical value of Aspergillus-specific IgG antibody test in the diagnosis of nonneutropenic invasive pulmonary aspergillosis","volume":"29","author":"Lu","year":"2023","journal-title":"Clin. Microbiol. Infect."},{"key":"B15","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1186\/s13054-020-03367-7","article-title":"Point of care aspergillus testing in intensive care patients","volume":"24","author":"Mercier","year":"2020","journal-title":"Crit. Care"},{"key":"B16","doi-asserted-by":"publisher","first-page":"1577","DOI":"10.1093\/cid\/ciaa295","article-title":"Prospective evaluation of the turbidimetric beta-D-glucan assay and 2 lateral flow assays on serum in invasive aspergillosis","volume":"72","author":"Mercier","year":"2021","journal-title":"Clin. Infect. Dis."},{"key":"B17","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1093\/mmy\/myv020","article-title":"Antibody testing in aspergillosis\u2013quo vadis","volume":"53","author":"Page","year":"2015","journal-title":"Med. Mycol"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00012","article-title":"Multicenter evaluation of a novel immunochromatographic test for anti-aspergillus IgG detection","volume":"9","author":"Piarroux","year":"2019","journal-title":"Front. Cell Infect. Microbiol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1183\/13993003.congress-2022.2719","article-title":"Efficacy of LD bio aspergillus ICT lateral flow assay for serodiagnosis of chronic pulmonary aspergillosis","volume":"8","author":"Ray","year":"2022","journal-title":"J. Fungi (Basel)"},{"key":"B20","doi-asserted-by":"publisher","first-page":"318","DOI":"10.3390\/jof6040318","article-title":"Chronic pulmonary aspergillosis in post tuberculosis patients in Indonesia and the role of LDBio aspergillus ICT as part of the diagnosis scheme","volume":"6","author":"Rozaliyani","year":"2020","journal-title":"J. Fungi (Basel)"},{"key":"B21","doi-asserted-by":"publisher","first-page":"2043","DOI":"10.2147\/IDR.S460513","article-title":"Value evaluation of quantitative aspergillus fumigatus-specific IgG antibody test in the diagnosis of non-neutropenic invasive pulmonary aspergillosis","volume":"17","author":"Sheng","year":"2024","journal-title":"Infect. Drug Resist."},{"key":"B22","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1016\/j.ijmmb.2022.03.002","article-title":"LDBio Aspergillus immunochromatographic test lateral flow assay for IgG\/IgM antibody detection in chronic pulmonary aspergillosis: Single-centre evaluation and meta-analysis","volume":"40","author":"Singh","year":"2022","journal-title":"Indian J. Med. Microbiol."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00538-19","article-title":"Evaluation of LDBio aspergillus ICT lateral flow assay for IgG and IgM antibody detection in chronic pulmonary aspergillosis","volume":"57","author":"Stucky Hunter","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"e1","DOI":"10.1016\/j.cmi.2018.01.002","article-title":"Diagnosis and management of Aspergillus diseases: executive summary of the 2017 ESCMID-ECMM-ERS guideline","author":"Ullmann","year":"2018","journal-title":"Clin. Microbiol. Infect."},{"key":"B25","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1186\/s12890-020-1125-y","article-title":"Potential value of serum Aspergillus IgG antibody detection in the diagnosis of invasive and chronic pulmonary aspergillosis in non-agranulocytic patients","volume":"20","author":"Yu","year":"2020","journal-title":"BMC Pulm Med."},{"key":"B26","doi-asserted-by":"publisher","first-page":"2153","DOI":"10.1128\/JCM.00345-17","article-title":"Diagnostic value of galactomannan antigen test in serum and bronchoalveolar lavage fluid samples from patients with nonneutropenic invasive pulmonary aspergillosis","volume":"55","author":"Zhou","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00264-23","article-title":"Diagnostic laboratory features and performance of an aspergillus IgG lateral flow assay in a chronic pulmonary aspergillosis cohort","volume":"11","author":"Zhu","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1271853","article-title":"Performance of mNGS in bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in non-neutropenic patients","volume":"13","author":"Zhu","year":"2023","journal-title":"Front. Cell Infect. Microbiol."}],"updated-by":[{"DOI":"10.3389\/fcimb.2025.1659574","type":"corrigendum","label":"Corrigendum","source":"publisher","updated":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000}}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2025.1599425\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T10:35:31Z","timestamp":1753439731000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2025.1599425\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,20]]},"references-count":28,"alternative-id":["10.3389\/fcimb.2025.1599425"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2025.1599425","relation":{"corrigendum":[{"id-type":"doi","id":"10.3389\/fcimb.2025.1659574","asserted-by":"object"}]},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,20]]},"article-number":"1599425"}}