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Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Hospital-acquired pneumonia (HAP) is a serious infection affecting patients in the hospital setting. This study aimed to evaluate a novel multiplex detection method using loop-mediated isothermal amplification (LAMP) technology to identify six primary bacterial pathogens responsible for HAP directly from respiratory samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 119 clinical samples were analyzed by LAMP technology, including mainly bronchoalveolar lavages, endotracheal aspirates, and bronchoaspirates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and discussion<\/jats:title>\n                    <jats:p>\n                      The results of the LAMP and traditional culture methods showed an accuracy of 93.0%. In some discordant cases between culture and LAMP, multiplex PCR (FilmArray Pneumonia Panel) showed a strong correlation with the LAMP results, confirming the potential use of this technique as a diagnostic detection tool. The clinical sensitivity of the LAMP assay was 93.3% with a specificity of 92.0%. Correlation analysis revealed a weak negative relationship between bacterial load and time to positivity (\n                      <jats:italic>r<\/jats:italic>\n                      = \u22120.177,\n                      <jats:italic>p<\/jats:italic>\n                      = 0.05). This study underscores the potential of LAMP as a rapid and accurate tool for the diagnosis of HAP, facilitating the turnaround time for microbiology laboratory results, which is critical for improving the outcomes of patients with HAP.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2025.1609666","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T05:59:26Z","timestamp":1757311166000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of a rapid diagnostic test based on loop-mediated isothermal amplification (LAMP) to identify the most frequent pathogens causing hospital-acquired pneumonia"],"prefix":"10.3389","volume":"15","author":[{"given":"Anna","family":"Sellar\u00e8s-Crous","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo","family":"Mart\u00ednez-Trejo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e0lia","family":"Arnalda-Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Gatti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Villanueva-L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Vergara-G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesc","family":"Marco-Revert\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateu","family":"Espasa-Soley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Vila-Estap\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.308.6943.1552","article-title":"Diagnostic tests. 1: Sensitivity and specificity","volume":"308","author":"Altman","year":"1994","journal-title":"BMJ"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3390\/v13050801","article-title":"Fast detection of SARS-coV-2 RNA directly from respiratory samples using a loop-mediated isothermal amplification (LAMP) test","volume":"13","author":"Anastasiou","year":"2021","journal-title":"Viruses"},{"key":"B3","doi-asserted-by":"publisher","first-page":"6722","DOI":"10.1038\/s41598-017-06503-6","article-title":"In-vivo loss of carbapenem resistance by extensively drug-resistant Klebsiella pneumoniae during treatment via porin expression modification","volume":"7","author":"Bialek-Davenet","year":"2017","journal-title":"Sci. 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