{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:31:04Z","timestamp":1790285464339,"version":"4.1.0"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"Objective<\/jats:title>This study aimed to investigate the correlation between alterations in the vaginal microecological environment and high-risk human papillomavirus (HR-HPV) infection, thereby providing a theoretical basis for clinical prevention and early intervention.<\/jats:p><\/jats:sec>Methods<\/jats:title>A total of 854 patients who visited the gynecology outpatient clinic at Wusong Central Hospital, Baoshan District, Shanghai, between June and December 2023 were included. Vaginal secretions were collected for HPV genotyping and microecological analysis. Patients were categorized into HR-HPV-positive (n=222) and HR-HPV-negative (n=632) groups. The detection rates of various HR-HPV subtypes (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) were analyzed. Key microecological indicators\u2014including vaginal pH, hydrogen peroxide, leukocyte esterase, sialidase, Lactobacillus<\/jats:italic> levels, and vaginal cleanliness\u2014were compared between the two groups, and their predictive value for HR-HPV infection was assessed using ROC curve analysis.<\/jats:p><\/jats:sec>Results<\/jats:title>Significant differences were observed between the two groups in terms of vaginal pH, hydrogen peroxide, leukocyte esterase, sialidase activity, Lactobacillus<\/jats:italic> abundance, and vaginal cleanliness (all P &lt; 0.05). The ROC curve analysis yielded an AUC of 0.701, indicating moderate predictive value.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Changes in the vaginal microecological environment are significantly associated with HR-HPV infection. Monitoring vaginal microecological indicators may provide adjunctive information for clinical prevention and early intervention, though further prospective studies are needed to establish causality.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2025.1618846","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T05:27:44Z","timestamp":1751520464000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Association between vaginal microecological alterations and high-risk human papillomavirus infection: a cross-sectional study"],"prefix":"10.3389","volume":"15","author":[{"given":"Nanqiu","family":"Peng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1177\/0272684X20916611","article-title":"Increasing human papillomavirus vaccination and cervical cancer screening in Nigeria: An assessment ofcommunity-based educational interventions","volume":"41","author":"Nkwonta","year":"2020","journal-title":"Int. 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