{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T18:28:04Z","timestamp":1784658484381,"version":"3.55.0"},"reference-count":22,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Recent studies have revealed that <jats:italic>Microviridae<\/jats:italic>, a family of ssDNA viruses, are widely distributed in natural environments and play significant roles in various ecosystems. While <jats:italic>Microviridae<\/jats:italic> members dominate the human gut microbiome, their genetic diversity in the human respiratory ecosystem remains unclear. The distribution, genetic characteristics, and ecological roles of <jats:italic>Microviridae<\/jats:italic> are still poorly understood. This study identified 327 Microviridae-associated contigs from nasopharyngeal swab samples of healthy individuals through metagenomic sequencing and comparative genomics analysis, including 15 near-complete <jats:italic>Microviridae<\/jats:italic>-related genomes. These genomes exhibited high sequence divergence from each other, revealing their high genetic diversity. Phylogenetic analysis based on VP1 (major capsid protein; F protein) demonstrated that the 15 genomes could be classified into seven distinct <jats:italic>Microviridae<\/jats:italic> groups. CRISPR spacer matching predicted the host of the 15 genomes. The total read counts of <jats:italic>Microviridae<\/jats:italic> across all 12 libraries were quantified and compared using the Kruskal-Wallis test. This work significantly expands the understanding of the diversity, genomic architecture, and evolutionary dynamics of <jats:italic>Microviridae<\/jats:italic> within the human respiratory tract.<\/jats:p>","DOI":"10.3389\/fcimb.2025.1629120","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T06:16:34Z","timestamp":1757312194000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Genetic diversity of Microviridae phages in the human respiratory tract"],"prefix":"10.3389","volume":"15","author":[{"given":"Peiting","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongfeng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1331","DOI":"10.1099\/jgv.0.001110","article-title":"Evaluation of the genomic diversity of viruses infecting bacteria, archaea and eukaryotes using a common bioinformatic platform: steps towards a unifed taxonomy","volume":"99","author":"Aiewsakun","year":"2018","journal-title":"J. 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