{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T21:22:10Z","timestamp":1774646530918,"version":"3.50.1"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To analyze the identification, antifungal resistance, and genomic characteristics of a <jats:italic>Candida auris<\/jats:italic> (<jats:italic>C. auris<\/jats:italic>) strain isolated from a urine specimen of an ICU patient at Yijishan Hospital, Wannan Medical College, Anhui Province.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The isolate was identified by Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). The susceptibility of the isolates to fungi was determined by measuring the Minimum Inhibitory Concentration (MIC) values using the VITEK 2 COMPACT system. Whole-genome sequencing (WGS) was performed using high-throughput technology. Resistance and virulence genes were annotated using public databases, including NCBI (<jats:ext-link>https:\/\/www.ncbi.nlm.nih.gov\/<\/jats:ext-link>, version 2.2.28), DFVF (<jats:ext-link>http:\/\/sysbio.unl.edu\/DFVF\/<\/jats:ext-link>, version 1.0), PHI-base (<jats:ext-link>http:\/\/www.phi-base.org<\/jats:ext-link>, version 5.0) and KEGG (<jats:ext-link>https:\/\/www.kegg.jp\/<\/jats:ext-link>, version 89.1). A phylogenetic tree was constructed through analysis of the 18S rRNA nucleotide sequence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The isolate named CAS20503 was identified as <jats:italic>C. auris<\/jats:italic>. Antifungal susceptibility testing showed resistance to fluconazole and amphotericin B. Genomic analysis identified resistance genes including <jats:italic>ERG11<\/jats:italic> (azole), <jats:italic>FKS1<\/jats:italic> (echinocandin), <jats:italic>ERG3<\/jats:italic> (polyene), and efflux pumps <jats:italic>CDR1<\/jats:italic>, <jats:italic>MDR1<\/jats:italic>. Resistance mutations were detected. The virulence genes analyzed based on the DFVF database included <jats:italic>CaNik1<\/jats:italic>, <jats:italic>CHS2<\/jats:italic>, <jats:italic>DUR1,2<\/jats:italic>, <jats:italic>HSP90<\/jats:italic>, <jats:italic>ICL1<\/jats:italic>, <jats:italic>PMT1<\/jats:italic>, <jats:italic>PMT2<\/jats:italic>, <jats:italic>PMT4<\/jats:italic>, <jats:italic>SSD1<\/jats:italic>, <jats:italic>TPS2<\/jats:italic>. The host pathogenic genes identified by comparison with the PHI-base database included <jats:italic>CaCHS1, ADE2, FAS2, PMR1, CaTPS2, Tfp1.<\/jats:italic> KEGG annotation showed enrichment in infectious disease pathways. The phylogenetic tree constncted based on the nudeotide sequence analysis of 18S rRNA indicated that this strain (Genome accession number: JBPYFS000000000)  exhibited a high degree of genomic similarity to the <jats:italic>C. auris<\/jats:italic> strain (Genome accession number: CP157510.1), which was isolated in ltaly in 2024 and belonged to clade l (a subset of the South Asian clade).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Through an in-depth analysis of strain CAS20503, which was isolated from a urinary tract infection specimen at a tertiary public hospital in Anhui Province (Yijishan Hospital, Wannan Medical College), this study elucidated the drug resistance profiles and genomic characteristics of <jats:italic>C. auris<\/jats:italic>. The findings have provided critical evidence for the early identification, diagnosis, and optimization of antifungal therapeutic regimens for infections caused by this pathogen in clinical practice.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcimb.2025.1641542","type":"journal-article","created":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T05:26:16Z","timestamp":1757481976000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification, antifungal resistance, and genomic characterization of a single Candida auris isolate from urinary tract infection"],"prefix":"10.3389","volume":"15","author":[{"given":"Jie","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangyan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoning","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1111\/myc.13445","article-title":"Expert recommendations for prevention and management of Candida auris transmission","volume":"65","author":"Aldejohann","year":"2022","journal-title":"Mycoses"},{"key":"B2","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00884-19","article-title":"Identification of Candida auris by use of the updated vitek 2 yeast identification system, version 8.01: a multilaboratory evaluation study","volume":"57","author":"Ambaraghassi","year":"2019","journal-title":"J. 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