{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:03:21Z","timestamp":1777629801168,"version":"3.51.4"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      The BACTEC MGIT 960 system is currently the most widely used rapid culture system for\n                      <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                      . However, its reagents have complex compositions, expensive, and rely on import. This study aimed to develop a self-formulated culture with simple composition, low cost, and performance comparable to or even better than that of the BACTEC MGIT 960 system.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Five experimental media were formulated by supplementing Sauton medium with carrot extract, potato extract, oleic acid, or choline chloride. These were compared against commercial MGIT 960 medium using\n                      <jats:italic>M. tuberculosis H37Rv<\/jats:italic>\n                      suspensions (10\n                      <jats:sup>-<\/jats:sup>\n                      \u00b3-10\n                      <jats:sup>-6<\/jats:sup>\n                      mg\/mL, triplicate cultures). Time to detection (TTD) and growth index (GI) were monitored automatically using the BACTEC MGIT 960 system.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      For medium-high inocula (10\n                      <jats:sup>-<\/jats:sup>\n                      \u00b3-10\n                      <jats:sup>-5<\/jats:sup>\n                      mg\/mL), carrot- and potato-supplemented Sauton media showed equivalent performance to MGIT 960 (1-day longer TTD, P&amp;gt;0.05; comparable GI values). At low inoculum (10\n                      <jats:sup>-6<\/jats:sup>\n                      mg\/mL), MGIT 960 failed to detect growth within 42 days, whereas supplemented Sauton medium achieved positivity within 30 days. The carrot extract medium performed best (mean TTD = 20.2 days), followed by oleic acid and oleic acid + choline chloride formulations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Carrot- and potato-supplemented Sauton medium demonstrated comparable performance to the MGIT 960 system for specimens with medium-to-high bacterial loads. Carrot extract, oleic acid, and choline chloride significantly enhanced the detection rate of low-inoculum, with carrot extract exhibiting the most pronounced growth-promoting effects. This study validates an accessible alternative to expensive imported media, particularly beneficial for resource-limited settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2025.1655595","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T07:12:44Z","timestamp":1762240364000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of five liquid culture media for rapid detection of Mycobacterium tuberculosis"],"prefix":"10.3389","volume":"15","author":[{"given":"Yihui","family":"Xie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoqin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxin","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zihui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueqin","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinzhong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,11,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"MGM2-0026-2013","DOI":"10.1128\/microbiolspec.MGM2-0026-2013","article-title":"Metabolomics of central carbon metabolism in mycobacterium tuberculosis","volume":"2","author":"Baughn","year":"2014","journal-title":"Microbiol. 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