{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T06:57:13Z","timestamp":1767769033761,"version":"3.48.0"},"reference-count":20,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017534","name":"Inner Mongolia Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017534","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Tacheng tick virus 1 (TcTV-1) is an emerging tick-borne nairovirus associated with human febrile illness. To date, no reliable detection method for TcTV-1 has been established. In this study, we developed and evaluated a rapid loop-mediated isothermal amplification (LAMP) assay for the detection of TcTV-1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The primers were designed based on the nucleocapsid protein (NP) gene of TcTV-1. Sensitivity was assessed using ten-fold serial dilutions of recombinant plasmids containing the target sequence. Specificity was evaluated using cDNA from Songling virus (SGLV), Yezo virus (YEZV), Tick-borne encephalitis virus (TBEV), Severe fever with thrombocytopenia syndrome virus (SFTSV), and Beiji nairovirus (BJNV). The assay was validated using field-collected tick samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The TcTV-1-specific LAMP assay detected as few as 1\u00d710\n                      <jats:sup>-1<\/jats:sup>\n                      copies\/\u03bcL within 60 minutes at 65\u202f\u00b0C and specifically amplified TcTV-1, with no cross-reactivity to SGLV, YEZV, TBEV, SFTSV, or BJNV. Positive reactions exhibited a clear color change from purple to blue, indicating a robust colorimetric response. A total of eight tick specimens (16.0%; 95% CI: 7.2\u201329.1) tested positive for TcTV-1 using both the established LAMP assay and SYBR Green real time quantitative polymerase chain reaction (RT-qPCR), demonstrating 100% sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy for the LAMP assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>We report a TcTV-1-specific LAMP assay with high sensitivity, specificity, and cost-effectiveness, making it a practical tool for use in field-based or resource-limited settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2025.1660327","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T06:52:15Z","timestamp":1767768735000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid and visual detection of Tacheng tick virus 1 using loop-mediated isothermal amplification technique"],"prefix":"10.3389","volume":"15","author":[{"given":"Qiqi","family":"Guo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Gui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanning","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziyan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zedong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingfeng","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1613","DOI":"10.1128\/jcm.06796-11","article-title":"Development of a loop-mediated isothermal amplification assay for rapid detection of capripoxviruses","volume":"50","author":"Das","year":"2012","journal-title":"J. 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