{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:41:41Z","timestamp":1788190901525,"version":"build-2803163510"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009329","name":"Scientific Research and Technology Development Program of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p><jats:italic>Aspergillus flavus<\/jats:italic> is a ubiquitous filamentous fungus that poses significant threats as both a causative agent of invasive aspergillosis and a major source of crop contamination due to production of aflatoxin B1 (AFB1). Sugars are essential for fungal metabolism, cell wall biosynthesis, and virulence, yet sugar transporters (STPs) in <jats:italic>A. flavus<\/jats:italic> remain largely uncharacterized. In this study, we systematically investigated three putative STP genes (<jats:italic>G4B84_001982<\/jats:italic>, <jats:italic>G4B84_005374<\/jats:italic>, and <jats:italic>G4B84_009351<\/jats:italic>) by comprehensive functional characterization of gene deletion mutants. Growth assays revealed that <jats:italic>G4B84_001982<\/jats:italic> and <jats:italic>G4B84_005374<\/jats:italic> mediate uptake of diverse sugar substrates, while <jats:italic>G4B84_009351<\/jats:italic> appeared to be non-essential under tested conditions. Heterologous expressions in the hexose transport-deficient <jats:italic>Saccharomyces cerevisiae<\/jats:italic> strain confirmed their sugar transporter activity. Phenotypic analysis revealed that the \u0394<jats:italic>1982<\/jats:italic> and \u0394<jats:italic>5374<\/jats:italic> mutants showed pleiotropic defects, including impaired growth, reduced sporulation, delayed germination, increased sensitivity to cell wall stressors, and completely abolished sclerotium formation. Pathogenicity assays demonstrated that the two mutants exhibited attenuated virulence in both plants (crop seeds) and animal (<jats:italic>Galleria mellonella<\/jats:italic>) infection model. Our findings highlight the essential of two STPs in <jats:italic>A. flavus<\/jats:italic> development, stress tolerance, and pathogenicity, offering insights into sugar-mediated pathogenicity in this economically and medically important fungus.<\/jats:p>","DOI":"10.3389\/fcimb.2025.1661799","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T05:59:01Z","timestamp":1757311141000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Key sugar transporters drive development and pathogenicity in Aspergillus flavus"],"prefix":"10.3389","volume":"15","author":[{"given":"Raheela","family":"Yasin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayed","family":"Usman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qijian","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiufang","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linqi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxia","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2029","DOI":"10.1099\/mic.0.26980-0","article-title":"Fungal cell wall chitinases and glucanases","volume":"150","author":"Adams","year":"2004","journal-title":"Microbiol. 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