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Recent advances in multi-omics technologies\u2014encompassing transcriptomics, proteomics, and metabolomics\u2014enhance our understanding of the molecular and cellular mechanisms driving liver fibrosis. This review integrates findings from human studies and animal models, highlighting key pathological pathways and their interactions. Multi-omics analyses also clarify therapeutic mechanisms targeting oxidative stress, inflammation, and metabolic dysfunction. Cross-species comparisons confirm the translational relevance of animal models and underscore the value of multi-omics approaches in biomarker discovery and precision therapy development. 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