{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T06:47:42Z","timestamp":1765954062108,"version":"3.48.0"},"reference-count":27,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Since 2019, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has posed a global health threat. Its high transmissibility and cross-species infectivity have disrupted public health systems and worldwide economies, with companion and agricultural animals, like cats and minks, showing high susceptibility. \u200bThis study evaluated rAd5-S6P, a recombinant vaccine using an adenovirus type 5 vector expressing a modified SARS-CoV-2 spike protein, in murine, feline, and mink models. Results demonstrated that rAd5-S6P elicited a robust humoral immune response when administered via intramuscular, intranasal, and oral routes, which conferred complete protection in mice. In feline and mink models, immunization with rAd-S6P resulted in significantly reduced viral shedding after high-dose SARS-CoV-2 challenge, and no infectious virus was detected in any of the examined organs of minks. Collectively, rAd5-S6P exhibited protective efficacy across species, supporting its translational potential as a veterinary vaccine. These findings provide critical evidence for animal vaccination strategies to control SARS-CoV-2 circulation and reduce zoonotic transmission risks.<\/jats:p>","DOI":"10.3389\/fcimb.2025.1714427","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T06:43:15Z","timestamp":1765953795000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the protective efficacy of a recombinant adenovirus-vectored SARS-CoV-2 vaccine candidate for veterinary use"],"prefix":"10.3389","volume":"15","author":[{"given":"Chong","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyuan","family":"Wen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gongxun","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Shuai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qilong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenyu","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinying","family":"Ge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xijun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinliang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renqiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianfeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuntao","family":"Guan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xijun","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigao","family":"Bu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines9060631","article-title":"A veterinary vaccine for SARS-CoV-2: the first COVID-19 vaccine for animals","volume":"9","author":"Chavda","year":"2021","journal-title":"Vaccines"},{"key":"B2","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1093\/eurpub\/ckab182","article-title":"SARS-CoV-2 outbreaks on Danish mink farms and mitigating public health interventions","volume":"32","author":"Dall Schmidt","year":"2022","journal-title":"Eur. 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